Detailed information of TA system TA056140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 241908..242427
• Replicon: chromosome
• Accession: NZ_CP012253
• Organism: Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AFK64_RS01220
• Protein ID: WP_004385271.1
• Coordinates: 242140..242427 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AFK64_RS01215
• Protein ID: WP_004385270.1
• Coordinates: 241908..242150 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK64_RS01200	237951..239069	+	1119	WP_004385267.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
AFK64_RS01205	239066..239806	+	741	WP_004385268.1	KDGP aldolase family protein	-
AFK64_RS01210	239920..241830	+	1911	WP_004385269.1	BglG family transcription antiterminator	-
AFK64_RS01215	241908..242150	+	243	WP_004385270.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AFK64_RS01220	242140..242427	+	288	WP_004385271.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK64_RS01225	242435..242899	-	465	WP_004385272.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
AFK64_RS01230	243284..245419	-	2136	WP_015386331.1	anaerobic ribonucleoside-triphosphate reductase	-
AFK64_RS01235	245716..246597	-	882	WP_007852197.1	LysR family transcriptional regulator	-
AFK64_RS01240	246700..247230	+	531	WP_004386036.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10811.80 Da
• Isoelectric Point: 10.5836

>T56140 WP_004385271.1 NZ_CP012253:242140-242427 [Cronobacter sakazakii]
MSYKLVFDPRALKEWHKLGETVKAQFKKKLAHVLAAPRVKSARLSGLPDCYKIKLRTSGYRLVYQVRDDAVCVLVIAIGK
RENLTVYQGAGHRLE

Nucleotide

• Length: 288 bp

>T56140 NZ_CP012253:242140-242427 [Cronobacter sakazakii]
ATGAGCTATAAACTGGTCTTTGACCCACGTGCGCTGAAGGAGTGGCACAAACTGGGCGAGACCGTTAAAGCGCAATTCAA
AAAGAAGCTGGCGCACGTGCTGGCGGCTCCCAGGGTGAAATCCGCCCGGTTAAGTGGCCTGCCGGACTGCTACAAAATTA
AATTAAGAACGAGCGGTTACCGGCTGGTGTATCAGGTGCGCGATGACGCTGTCTGTGTGCTGGTCATCGCGATCGGCAAA
CGCGAAAACTTGACCGTATACCAGGGTGCCGGGCACCGTCTGGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9037.45 Da
• Isoelectric Point: 5.1088

>AT56140 WP_004385270.1 NZ_CP012253:241908-242150 [Cronobacter sakazakii]
MATINVRLDDKLKNEAYAVLEKLGITPTEAVRLLFQYVAQNGRMPVKTLTVSDSEEALLRTVRERLADPRPGIRVDLDEL

Nucleotide

• Length: 243 bp

>AT56140 NZ_CP012253:241908-242150 [Cronobacter sakazakii]
ATGGCCACAATCAACGTAAGGCTTGATGACAAGCTTAAAAATGAGGCTTATGCGGTGCTGGAAAAACTGGGTATCACACC
TACCGAAGCGGTGCGTCTGCTGTTTCAGTATGTGGCACAAAATGGCCGTATGCCGGTCAAAACGCTAACCGTCAGCGACA
GCGAAGAGGCGCTGCTGCGTACCGTGCGTGAGCGACTGGCGGACCCGCGGCCGGGCATCAGGGTTGATCTGGATGAGCTA
TAA