Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 43546..44131 Replicon chromosome
Accession NZ_CP012253
Organism Cronobacter sakazakii strain NCTC 8155

Toxin (Protein)


Gene name PumA Uniprot ID A0A853H7C5
Locus tag AFK64_RS00275 Protein ID WP_014727945.1
Coordinates 43826..44131 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A2S9UB62
Locus tag AFK64_RS00270 Protein ID WP_007852536.1
Coordinates 43546..43821 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK64_RS00265 41898..43529 + 1632 WP_007852537.1 Na/Pi cotransporter family protein -
AFK64_RS00270 43546..43821 - 276 WP_007852536.1 putative addiction module antidote protein Antitoxin
AFK64_RS00275 43826..44131 - 306 WP_014727945.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK64_RS00280 44348..44698 + 351 WP_007852532.1 type II toxin-antitoxin system RelE/ParE family toxin -
AFK64_RS00285 44695..45018 + 324 WP_015386308.1 helix-turn-helix domain-containing protein -
AFK64_RS00290 45045..45317 - 273 WP_004387517.1 DUF3811 domain-containing protein -
AFK64_RS00295 45408..46343 - 936 WP_004387518.1 ketopantoate/pantoate/pantothenate transporter PanS -
AFK64_RS00300 46505..48097 - 1593 WP_004387519.1 RNA repair transcriptional activator RtcR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-89)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11588.58 Da        Isoelectric Point: 10.3395

>T56138 WP_014727945.1 NZ_CP012253:c44131-43826 [Cronobacter sakazakii]
MKHIFQTEVFRRWQASLKDQRVKTMIAARLFRLANGLPGDVKPVGAGISELRIHYGAGYRIYFKQHGEVIVILLCGGDKH
TQDKDILLAKMLADLPENQWR

Download         Length: 306 bp

>T56138 NZ_CP012253:c44131-43826 [Cronobacter sakazakii]
ATGAAGCATATTTTTCAAACTGAAGTATTCCGCCGCTGGCAAGCCAGTCTGAAAGACCAGCGGGTAAAAACGATGATTGC
CGCGCGGCTTTTTCGGCTGGCTAATGGTTTGCCTGGTGATGTTAAGCCCGTCGGCGCAGGCATCAGTGAACTGCGTATTC
ACTACGGCGCGGGATACCGGATTTATTTCAAACAACACGGCGAGGTGATTGTCATTTTGCTTTGTGGTGGTGATAAGCAC
ACGCAGGATAAAGACATTCTTCTTGCGAAAATGCTGGCTGACCTGCCAGAGAACCAGTGGAGATAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9597.06 Da        Isoelectric Point: 6.4609

>AT56138 WP_007852536.1 NZ_CP012253:c43821-43546 [Cronobacter sakazakii]
MKLTEYDPAAALVDDEEIAFFMADALETGDAGHIAKALGVVARAKGMSHIAAQTGLSREQLYRSFSDKGNPTLKTTLAVM
KALGLGLTVKS

Download         Length: 276 bp

>AT56138 NZ_CP012253:c43821-43546 [Cronobacter sakazakii]
ATGAAGCTGACCGAATACGATCCCGCAGCGGCGCTGGTAGATGATGAGGAGATCGCCTTCTTTATGGCGGACGCGCTCGA
AACTGGCGACGCCGGACATATTGCAAAGGCGCTGGGCGTCGTCGCGCGCGCTAAAGGCATGTCGCATATCGCCGCTCAGA
CAGGGCTGTCGCGTGAACAGCTGTACCGTTCTTTCAGTGATAAAGGCAACCCAACGCTTAAAACCACGCTGGCAGTGATG
AAAGCGTTGGGTCTCGGTTTAACGGTTAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853H7C5

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UB62

References