Detailed information of TA system TA056118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3169991..3170610
• Replicon: chromosome
• Accession: NZ_CP012201
• Organism: Massilia sp. NR 4-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ACZ75_RS27570
• Protein ID: WP_082219507.1
• Coordinates: 3170428..3170610 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0K1JZ25
• Locus tag: ACZ75_RS12870
• Protein ID: WP_050409107.1
• Coordinates: 3169991..3170416 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACZ75_RS12860	3164999..3165778	+	780	WP_050409106.1	trypsin-like serine protease	-
ACZ75_RS27565	3165965..3169948	+	3984	WP_082219506.1	FAD-dependent oxidoreductase	-
ACZ75_RS12870	3169991..3170416	-	426	WP_050409107.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ACZ75_RS27570	3170428..3170610	-	183	WP_082219507.1	type II toxin-antitoxin system HicA family toxin	Toxin
ACZ75_RS27575	3170930..3171130	+	201	WP_082219508.1	DUF3079 domain-containing protein	-
ACZ75_RS27580	3171640..3172074	-	435	WP_082219509.1	TonB family protein	-
ACZ75_RS12890	3172648..3174645	-	1998	WP_050409110.1	acetate--CoA ligase	-
ACZ75_RS12895	3174900..3175508	+	609	WP_050409111.1	YqhA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6882.04 Da
• Isoelectric Point: 11.9537

>T56118 WP_082219507.1 NZ_CP012201:c3170610-3170428 [Massilia sp. NR 4-1]
MNSKTLIDMLKADGWQLVRIRGSHHHFRHPGKNGLITVPHPKKDLPAGTWRRILHDAGLR

Nucleotide

• Length: 183 bp

>T56118 NZ_CP012201:c3170610-3170428 [Massilia sp. NR 4-1]
ATGAACTCCAAGACCTTAATTGATATGCTGAAGGCAGATGGATGGCAGCTTGTACGGATCAGAGGCAGCCATCATCATTT
CAGGCATCCTGGAAAGAATGGTTTGATTACCGTGCCGCATCCCAAGAAGGATTTACCAGCAGGGACGTGGCGTCGCATCT
TGCATGATGCTGGGTTGAGGTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15632.76 Da
• Isoelectric Point: 4.6257

>AT56118 WP_050409107.1 NZ_CP012201:c3170416-3169991 [Massilia sp. NR 4-1]
MDLPVIIFKDEGSVYGVSVPDISGCHSWGDTIGDALRNVKEAIASHIETLLELGENIEVTPSDIECLRERPEYEGAIWAL
VNVDLSKLDSKPERINISLPRFVLSKIDKYADERHETRSGLLARAAVKLINDETALKTSKT

Nucleotide

• Length: 426 bp

>AT56118 NZ_CP012201:c3170416-3169991 [Massilia sp. NR 4-1]
ATGGATCTTCCGGTCATAATTTTTAAGGATGAAGGCAGCGTATATGGCGTGAGCGTGCCGGATATCAGCGGCTGCCATTC
ATGGGGAGACACGATTGGTGACGCGCTTCGCAACGTGAAAGAGGCCATTGCCAGTCACATCGAAACGCTGCTGGAGCTTG
GCGAAAACATTGAGGTGACGCCGTCGGATATTGAGTGTTTGCGTGAGCGTCCGGAATACGAAGGCGCTATCTGGGCGCTT
GTGAATGTCGATCTGAGCAAGCTTGATAGCAAACCTGAGAGGATCAATATCAGCTTGCCCCGCTTTGTCCTCAGCAAGAT
CGATAAGTACGCAGATGAACGTCATGAAACCCGCAGCGGTTTGCTGGCGCGGGCTGCCGTAAAACTGATTAACGACGAAA
CGGCCCTGAAAACAAGCAAAACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K1JZ25