56117

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 947939..949659 Replicon chromosome
Accession NZ_CP012201
Organism Massilia sp. NR 4-1

Toxin (Protein)


Gene name pmenT Uniprot ID A0A0K1JUY9
Locus tag ACZ75_RS03890 Protein ID WP_050407516.1
Coordinates 947939..948583 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A0K1JUI6
Locus tag ACZ75_RS03895 Protein ID WP_050407517.1
Coordinates 948583..949659 (+) Length 359 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACZ75_RS03865 943136..943384 + 249 WP_050407511.1 hypothetical protein -
ACZ75_RS03870 943371..945020 + 1650 WP_150118975.1 hypothetical protein -
ACZ75_RS03875 945866..946447 + 582 WP_050407513.1 hypothetical protein -
ACZ75_RS03880 946494..946874 + 381 WP_050407514.1 hypothetical protein -
ACZ75_RS27995 946958..947281 + 324 WP_150118976.1 helix-turn-helix domain-containing protein -
ACZ75_RS26980 947370..947939 + 570 WP_082219303.1 DUF4433 domain-containing protein -
ACZ75_RS03890 947939..948583 + 645 WP_050407516.1 DUF4433 domain-containing protein Toxin
ACZ75_RS03895 948583..949659 + 1077 WP_050407517.1 macro domain-containing protein Antitoxin
ACZ75_RS03900 950139..950966 - 828 WP_082219304.1 HEPN domain-containing protein -
ACZ75_RS03905 950959..951462 - 504 WP_050412337.1 hypothetical protein -
ACZ75_RS26985 951474..953165 - 1692 WP_190287760.1 ADP-ribosylglycohydrolase family protein -
ACZ75_RS26990 953183..953845 - 663 WP_082219306.1 DUF4433 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 937276..992423 55147


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-214)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24899.56 Da        Isoelectric Point: 7.4697

>T56117 WP_050407516.1 NZ_CP012201:947939-948583 [Massilia sp. NR 4-1]
MLYNNLNPEKALIWRIVHRDNLSWILDNGLYCGNSEVKAPAWVNIGNPELIDKRARHSVPLPPKGLLNDYVPFYFTPFSP
MLRNIHTGWGGIKKRPNEEIVILVSSLRHIAGLGLPFLFTDSHAYYQWADYYSDLADLDKIDWPLLQRRDFKRDPEDPAK
FERYQAEALIHQHVPVGGLLGIVCYTEPLKQRIEQEIQARNLNLPVYARTGWYF

Download         Length: 645 bp

>T56117 NZ_CP012201:947939-948583 [Massilia sp. NR 4-1]
ATGCTTTATAACAATCTCAATCCGGAAAAGGCACTGATCTGGCGGATTGTTCATCGTGACAACCTTTCGTGGATTCTGGA
TAATGGCCTATATTGTGGCAATAGCGAGGTGAAGGCACCAGCTTGGGTGAATATCGGGAACCCGGAACTTATCGACAAGC
GGGCTCGTCATTCAGTGCCGCTGCCGCCGAAAGGCTTGCTGAATGATTATGTGCCATTCTACTTCACGCCTTTTTCGCCA
ATGCTGCGCAACATCCACACGGGATGGGGTGGGATCAAAAAGCGGCCGAACGAAGAAATCGTGATCTTGGTTTCCAGCCT
TCGTCACATTGCCGGACTCGGATTGCCGTTTCTTTTCACAGACAGCCATGCTTATTATCAGTGGGCAGATTATTATTCAG
ACTTGGCCGACCTCGATAAAATCGACTGGCCTCTGTTGCAGCGAAGGGATTTCAAGCGAGACCCGGAGGACCCTGCCAAG
TTTGAGCGATACCAGGCCGAGGCACTAATTCACCAACACGTGCCAGTTGGAGGGCTTCTTGGCATCGTGTGTTATACAGA
ACCTTTGAAACAACGCATCGAACAGGAGATTCAGGCTCGTAATTTGAATCTACCGGTTTATGCCCGAACAGGGTGGTACT
TTTAA

Antitoxin


Download         Length: 359 a.a.        Molecular weight: 40457.29 Da        Isoelectric Point: 5.3232

>AT56117 WP_050407517.1 NZ_CP012201:948583-949659 [Massilia sp. NR 4-1]
MITFTQGNLLEARAEALVNTVNTVGVMGKGIALMFKERFDENFLRYAAACKAKEVQTGKMFVTSVHELDGPRWIVNFPTK
QHWRAPSRMEWVVEGLQDLRRFLIEQQIQSIAIPPLGAGNGGLEWGDVREQIELALGDLDIDILVFEPTKQYQNVAKRAG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFVERAIERYNPGDNPLNLQFTAHKYGPYANRLDHLLNNLDGSYLH
SEKRISDADPLDVIWFDDERKAFVQAYLNSEAKAYTQALESTAALINGFESPFGMELLATVDWLLAREGVEPNVPALREG
LRHWRGGSDAAMRKDRLFDDRALGIALERLTQPATAMA

Download         Length: 1077 bp

>AT56117 NZ_CP012201:948583-949659 [Massilia sp. NR 4-1]
ATGATTACATTCACTCAAGGCAATTTGTTGGAGGCCCGCGCCGAGGCGCTGGTTAACACTGTCAACACCGTCGGGGTGAT
GGGCAAAGGCATCGCGCTGATGTTTAAGGAGCGGTTTGATGAGAATTTCCTCCGCTATGCTGCCGCCTGTAAAGCCAAGG
AAGTGCAGACTGGCAAAATGTTTGTTACGTCGGTGCATGAATTGGATGGGCCGCGTTGGATCGTCAACTTCCCCACTAAG
CAGCACTGGAGGGCTCCATCACGCATGGAGTGGGTGGTAGAAGGCTTGCAGGACTTGCGGCGCTTCTTGATTGAGCAACA
GATTCAATCCATCGCCATTCCGCCGTTGGGTGCTGGTAATGGAGGGCTGGAATGGGGGGATGTGCGTGAACAAATTGAAT
TGGCGTTAGGTGATCTGGACATAGATATCTTGGTGTTTGAGCCGACTAAGCAATACCAAAATGTGGCCAAGCGAGCCGGC
GTAGAAAAGCTGACGCCGGCTCGTGCACTGATTGCCGAACTTGTGCGCCGGTACTGGGTGTTGGGCATGGAATGCAGTCT
GTTGGAGATTCAAAAGTTGGCATGGTTTGTGGAGCGCGCTATCGAGCGCTACAACCCTGGCGACAACCCTCTGAATCTGC
AATTCACGGCCCATAAATACGGTCCTTACGCCAATCGACTTGATCACCTATTGAACAACTTGGATGGAAGCTATCTGCAC
TCCGAAAAACGAATCAGCGATGCTGACCCGCTGGACGTGATTTGGTTTGACGATGAACGCAAAGCTTTTGTCCAAGCCTA
CCTCAACAGTGAGGCCAAGGCATACACTCAAGCCCTGGAATCGACGGCTGCATTGATCAACGGTTTTGAATCACCCTTCG
GCATGGAATTGTTGGCGACTGTGGATTGGCTGCTTGCTCGTGAAGGTGTGGAACCCAATGTACCGGCTCTACGCGAAGGG
CTCCGCCATTGGCGGGGCGGCTCGGATGCTGCCATGCGTAAGGACCGACTCTTCGATGATCGGGCACTAGGCATTGCCCT
GGAGCGCTTGACACAACCCGCGACAGCAATGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K1JUY9

Antitoxin

Source ID Structure
AlphaFold DB A0A0K1JUI6

References