Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 90271..90905 Replicon plasmid pBCI1-1
Accession NZ_CP012186
Organism Pseudonocardia sp. EC080619-01

Toxin (Protein)


Gene name vapC Uniprot ID A0A0P0SM87
Locus tag AD017_RS32575 Protein ID WP_060575006.1
Coordinates 90271..90690 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P0SNA2
Locus tag AD017_RS32580 Protein ID WP_060576479.1
Coordinates 90681..90905 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD017_RS32550 85776..86966 - 1191 WP_145986132.1 IS701 family transposase -
AD017_RS32560 87062..88326 + 1265 WP_145986114.1 IS3 family transposase -
AD017_RS32565 88253..88660 + 408 WP_082539132.1 hypothetical protein -
AD017_RS32570 88657..89748 - 1092 WP_060575005.1 IS630 family transposase -
AD017_RS32575 90271..90690 - 420 WP_060575006.1 PIN domain nuclease Toxin
AD017_RS32580 90681..90905 - 225 WP_060576479.1 hypothetical protein Antitoxin
AD017_RS32590 91416..92626 + 1211 WP_145982879.1 IS3 family transposase -
AD017_RS32595 92788..93150 + 363 WP_060575009.1 hypothetical protein -
AD017_RS32600 93190..93546 + 357 WP_082399689.1 maleylpyruvate isomerase N-terminal domain-containing protein -
AD017_RS34160 94108..94482 + 375 Protein_100 helix-turn-helix domain containing protein -
AD017_RS32605 94517..95464 - 948 Protein_101 IS701 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..206535 206535


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(29-127)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15252.58 Da        Isoelectric Point: 5.9251

>T56110 WP_060575006.1 NZ_CP012186:c90690-90271 [Pseudonocardia sp. EC080619-01]
VAVTSWLVDKSAYERMQSDRAAGMDEWSARVERGLLRLSTITRLELGFSARSGRSGREAFLRTPLSLMPIERLTPAMEDR
AFEVQMLLADRGQHRAPSIPDLLIAATAEKAGLTVLAIDKDFDLIAKVTGQPVETLAAV

Download         Length: 420 bp

>T56110 NZ_CP012186:c90690-90271 [Pseudonocardia sp. EC080619-01]
GTGGCGGTGACGAGCTGGCTCGTTGACAAGTCCGCCTACGAGCGGATGCAGTCGGACCGGGCCGCTGGCATGGACGAGTG
GAGCGCGCGCGTCGAGCGTGGCCTACTGCGGCTGTCGACGATCACGCGGCTTGAGCTGGGCTTCTCCGCGCGGTCGGGTA
GGTCGGGACGCGAGGCGTTCCTCAGGACGCCCCTGTCGCTTATGCCCATCGAACGCCTCACTCCGGCGATGGAGGACCGG
GCATTTGAGGTCCAGATGCTGCTCGCCGACCGAGGTCAGCATCGCGCCCCGTCGATCCCGGACCTGCTTATCGCGGCGAC
AGCCGAGAAAGCAGGGCTCACGGTGCTCGCCATTGATAAGGACTTCGACCTCATTGCAAAGGTCACGGGGCAACCGGTGG
AGACACTCGCTGCAGTGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8062.94 Da        Isoelectric Point: 4.1068

>AT56110 WP_060576479.1 NZ_CP012186:c90905-90681 [Pseudonocardia sp. EC080619-01]
MTNVLIRGLSDAAVERIDAAASELGLSRNEFLRRKLEAGTPPSAESELTAEDWARSAEVFGDLSDPEVMDAAWR

Download         Length: 225 bp

>AT56110 NZ_CP012186:c90905-90681 [Pseudonocardia sp. EC080619-01]
ATGACGAACGTGCTCATCAGAGGGCTCAGCGACGCGGCGGTGGAGCGCATCGACGCGGCGGCGTCCGAGCTTGGCTTGTC
CCGCAACGAGTTCCTGCGCCGCAAGCTCGAAGCCGGTACGCCTCCGAGTGCCGAGAGCGAGCTCACGGCCGAGGACTGGG
CCCGCTCGGCAGAGGTGTTCGGAGACCTGTCCGATCCCGAGGTGATGGACGCCGCGTGGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0SM87

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SNA2

References