Detailed information of TA system TA056109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4091774..4092408
• Replicon: chromosome
• Accession: NZ_CP012184
• Organism: Pseudonocardia sp. EC080619-01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0P0SM87
• Locus tag: AD017_RS19140
• Protein ID: WP_060575006.1
• Coordinates: 4091774..4092193 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0P0SNA2
• Locus tag: AD017_RS19145
• Protein ID: WP_060576479.1
• Coordinates: 4092184..4092408 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD017_RS35835	4087383..4087623	+	241	Protein_3805	potassium-transporting ATPase subunit KdpA	-
AD017_RS35840	4087803..4087995	+	193	Protein_3806	winged helix-turn-helix domain-containing protein	-
AD017_RS35385	4088029..4088190	-	162	WP_168170448.1	hypothetical protein	-
AD017_RS19120	4088479..4089015	+	537	WP_029239649.1	glyoxalase	-
AD017_RS19125	4089312..4089527	-	216	WP_060575003.1	hypothetical protein	-
AD017_RS19130	4089548..4089919	-	372	WP_075301941.1	hypothetical protein	-
AD017_RS19135	4090160..4091251	-	1092	WP_060575005.1	IS630 family transposase	-
AD017_RS19140	4091774..4092193	-	420	WP_060575006.1	PIN domain nuclease	Toxin
AD017_RS19145	4092184..4092408	-	225	WP_060576479.1	hypothetical protein	Antitoxin
AD017_RS19155	4092919..4094129	+	1211	WP_145982879.1	IS3 family transposase	-
AD017_RS19160	4094291..4094653	+	363	WP_060575009.1	hypothetical protein	-
AD017_RS19165	4094693..4095049	+	357	WP_082399689.1	maleylpyruvate isomerase N-terminal domain-containing protein	-
AD017_RS33520	4095611..4095985	+	375	Protein_3817	helix-turn-helix domain containing protein	-
AD017_RS19170	4096020..4096967	-	948	Protein_3818	IS701 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4081274..4099944	18670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (29-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15252.58 Da
• Isoelectric Point: 5.9251

>T56109 WP_060575006.1 NZ_CP012184:c4092193-4091774 [Pseudonocardia sp. EC080619-01]
VAVTSWLVDKSAYERMQSDRAAGMDEWSARVERGLLRLSTITRLELGFSARSGRSGREAFLRTPLSLMPIERLTPAMEDR
AFEVQMLLADRGQHRAPSIPDLLIAATAEKAGLTVLAIDKDFDLIAKVTGQPVETLAAV

Nucleotide

• Length: 420 bp

>T56109 NZ_CP012184:c4092193-4091774 [Pseudonocardia sp. EC080619-01]
GTGGCGGTGACGAGCTGGCTCGTTGACAAGTCCGCCTACGAGCGGATGCAGTCGGACCGGGCCGCTGGCATGGACGAGTG
GAGCGCGCGCGTCGAGCGTGGCCTACTGCGGCTGTCGACGATCACGCGGCTTGAGCTGGGCTTCTCCGCGCGGTCGGGTA
GGTCGGGACGCGAGGCGTTCCTCAGGACGCCCCTGTCGCTTATGCCCATCGAACGCCTCACTCCGGCGATGGAGGACCGG
GCATTTGAGGTCCAGATGCTGCTCGCCGACCGAGGTCAGCATCGCGCCCCGTCGATCCCGGACCTGCTTATCGCGGCGAC
AGCCGAGAAAGCAGGGCTCACGGTGCTCGCCATTGATAAGGACTTCGACCTCATTGCAAAGGTCACGGGGCAACCGGTGG
AGACACTCGCTGCAGTGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8062.94 Da
• Isoelectric Point: 4.1068

>AT56109 WP_060576479.1 NZ_CP012184:c4092408-4092184 [Pseudonocardia sp. EC080619-01]
MTNVLIRGLSDAAVERIDAAASELGLSRNEFLRRKLEAGTPPSAESELTAEDWARSAEVFGDLSDPEVMDAAWR

Nucleotide

• Length: 225 bp

>AT56109 NZ_CP012184:c4092408-4092184 [Pseudonocardia sp. EC080619-01]
ATGACGAACGTGCTCATCAGAGGGCTCAGCGACGCGGCGGTGGAGCGCATCGACGCGGCGGCGTCCGAGCTTGGCTTGTC
CCGCAACGAGTTCCTGCGCCGCAAGCTCGAAGCCGGTACGCCTCCGAGTGCCGAGAGCGAGCTCACGGCCGAGGACTGGG
CCCGCTCGGCAGAGGTGTTCGGAGACCTGTCCGATCCCGAGGTGATGGACGCCGCGTGGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0P0SM87

Antitoxin

• AlphaFold DB: A0A0P0SNA2