Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 2648..3262 Replicon plasmid unnamed
Accession NZ_CP012180
Organism Pseudomonas syringae pv. actinidiae ICMP 18708

Toxin (Protein)


Gene name PfiT Uniprot ID A0A2V0R8Y7
Locus tag NZ708_RS29380 Protein ID WP_017685123.1
Coordinates 2912..3262 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID S6SUK0
Locus tag NZ708_RS29375 Protein ID WP_017685122.1
Coordinates 2648..2896 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZ708_RS29365 80..1147 + 1068 WP_074556923.1 IS630 family transposase -
NZ708_RS29370 1345..2307 - 963 WP_017685121.1 tyrosine-type recombinase/integrase -
NZ708_RS29375 2648..2896 + 249 WP_017685122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
NZ708_RS29380 2912..3262 + 351 WP_017685123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NZ708_RS32885 3394..3978 - 585 Protein_4 helix-turn-helix transcriptional regulator -
NZ708_RS29405 5685..5990 + 306 WP_017706770.1 type IV secretion system protein -
NZ708_RS29410 5980..6789 + 810 WP_017684942.1 P-type conjugative transfer protein VirB9 -
NZ708_RS29415 6776..8146 + 1371 WP_017684943.1 TrbI/VirB10 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74432 74432
- inside IScluster/Tn - - 416..5528 5112


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13012.89 Da        Isoelectric Point: 4.1953

>T56107 WP_017685123.1 NZ_CP012180:2912-3262 [Pseudomonas syringae pv. actinidiae ICMP 18708]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T56107 NZ_CP012180:2912-3262 [Pseudomonas syringae pv. actinidiae ICMP 18708]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9389.72 Da        Isoelectric Point: 9.5019

>AT56107 WP_017685122.1 NZ_CP012180:2648-2896 [Pseudomonas syringae pv. actinidiae ICMP 18708]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT56107 NZ_CP012180:2648-2896 [Pseudomonas syringae pv. actinidiae ICMP 18708]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0R8Y7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0R8I6

References