Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 741446..742119 Replicon chromosome
Accession NZ_CP012179
Organism Pseudomonas syringae pv. actinidiae ICMP 18708

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag NZ708_RS03580 Protein ID WP_017684243.1
Coordinates 741446..741865 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag NZ708_RS03585 Protein ID WP_003381346.1
Coordinates 741862..742119 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZ708_RS03555 736456..737490 - 1035 WP_017684240.1 sulfotransferase -
NZ708_RS03560 737508..738428 - 921 WP_020313563.1 TauD/TfdA family dioxygenase -
NZ708_RS03565 738574..739221 - 648 WP_017703697.1 histidine phosphatase family protein -
NZ708_RS03570 739562..740497 - 936 WP_017704392.1 syringolide biosynthetic protein AvrD1 -
NZ708_RS03575 740772..741405 - 634 Protein_698 recombinase family protein -
NZ708_RS03580 741446..741865 - 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
NZ708_RS03585 741862..742119 - 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
NZ708_RS03590 742474..743130 + 657 WP_017684244.1 hypothetical protein -
NZ708_RS03595 743178..743432 + 255 WP_003381350.1 hypothetical protein -
NZ708_RS30100 744249..745211 - 963 WP_003383614.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 729368..788199 58831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T56102 WP_017684243.1 NZ_CP012179:c741865-741446 [Pseudomonas syringae pv. actinidiae ICMP 18708]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T56102 NZ_CP012179:c741865-741446 [Pseudomonas syringae pv. actinidiae ICMP 18708]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGATCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT56102 WP_003381346.1 NZ_CP012179:c742119-741862 [Pseudomonas syringae pv. actinidiae ICMP 18708]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT56102 NZ_CP012179:c742119-741862 [Pseudomonas syringae pv. actinidiae ICMP 18708]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References