Detailed information of TA system TA056099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-AbrB
• Location: 1286428..1287074
• Replicon: chromosome
• Accession: NZ_CP012176
• Organism: Metallosphaera sedula strain SARC-M1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A088E7Y6
• Locus tag: MsedE_RS06635
• Protein ID: WP_012021252.1
• Coordinates: 1286428..1286817 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MsedE_RS06640
• Protein ID: WP_012021253.1
• Coordinates: 1286814..1287074 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MsedE_RS06615 (MsedE_1327)	1282164..1283345	+	1182	WP_012021248.1	mandelate racemase/muconate lactonizing enzyme family protein	-
MsedE_RS06620 (MsedE_1328)	1283414..1284037	-	624	WP_012021249.1	PIG-L family deacetylase	-
MsedE_RS06625 (MsedE_1329)	1284072..1285115	-	1044	WP_012021250.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
MsedE_RS06630 (MsedE_1330)	1285208..1286371	-	1164	WP_012021251.1	glycosyltransferase	-
MsedE_RS06635 (MsedE_1331)	1286428..1286817	-	390	WP_012021252.1	type II toxin-antitoxin system VapC family toxin	Toxin
MsedE_RS06640 (MsedE_1332)	1286814..1287074	-	261	WP_012021253.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MsedE_RS06645 (MsedE_1333)	1287140..1287766	-	627	WP_048060318.1	type 1 glutamine amidotransferase	-
MsedE_RS06650 (MsedE_1334)	1287995..1288645	+	651	WP_012021255.1	cyclase family protein	-
MsedE_RS06655 (MsedE_1335)	1288647..1289156	+	510	WP_012021256.1	OsmC family protein	-
MsedE_RS06660 (MsedE_1336)	1289342..1290694	+	1353	WP_012021257.1	APC family permease	-
MsedE_RS06665 (MsedE_1337)	1290709..1291281	-	573	WP_012021258.1	hypothetical protein	-
MsedE_RS06670 (MsedE_1338)	1291513..1291965	+	453	WP_012021259.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15075.63 Da
• Isoelectric Point: 4.9164

>T56099 WP_012021252.1 NZ_CP012176:c1286817-1286428 [Metallosphaera sedula]
MIFLDANFLVYLNLGVDPVKSFYLRILSNESMGVDPLVLDEVIYVSRKKYGVNFGDTLRFLDELVLPYVVVFPISLREYQ
VSKELITRYSLYPSDAFHVAVMLNNSIKKILTEDSDFEKIKEIERIWIK

Nucleotide

• Length: 390 bp

>T56099 NZ_CP012176:c1286817-1286428 [Metallosphaera sedula]
TTGATATTCTTGGATGCAAACTTCTTGGTGTACCTAAACCTGGGAGTTGACCCGGTCAAGAGCTTCTACCTCAGGATACT
TTCCAATGAGAGTATGGGCGTTGACCCCCTGGTCCTAGACGAGGTGATCTACGTCTCGAGGAAGAAGTATGGCGTGAATT
TCGGCGACACATTGCGATTTCTGGACGAGCTAGTCTTACCTTACGTAGTGGTATTTCCGATCTCCCTGAGGGAGTACCAG
GTTAGCAAGGAGCTCATCACTAGGTATTCCCTCTATCCCTCAGACGCGTTTCACGTGGCAGTAATGCTCAACAACTCCAT
AAAGAAGATTCTCACCGAGGACTCCGACTTCGAGAAGATCAAGGAAATAGAGAGGATCTGGATAAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10073.56 Da
• Isoelectric Point: 4.8329

>AT56099 WP_012021253.1 NZ_CP012176:c1287074-1286814 [Metallosphaera sedula]
MTLRVEVGKKGYMIIPKNVRDLLGINEGDFLELRVENGKIVLEKERKVDLEEVERKFEEHERRIAYARRASLGDLKGVVL
EEEFDD

Nucleotide

• Length: 261 bp

>AT56099 NZ_CP012176:c1287074-1286814 [Metallosphaera sedula]
TTGACTCTTAGGGTAGAGGTAGGAAAGAAGGGTTACATGATAATCCCCAAGAACGTCAGAGACCTGCTCGGCATCAATGA
GGGCGACTTCCTGGAACTCAGGGTAGAGAATGGCAAGATAGTCCTGGAAAAGGAAAGGAAAGTTGATCTGGAGGAGGTGG
AGAGGAAGTTTGAGGAACACGAGAGAAGGATCGCGTATGCAAGAAGGGCCAGCCTTGGAGATTTAAAGGGCGTCGTGTTG
GAGGAGGAGTTTGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A088E7Y6