Detailed information of TA system TA056096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-AbrB
• Location: 1286424..1287070
• Replicon: chromosome
• Accession: NZ_CP012173
• Organism: Metallosphaera sedula strain ARS50-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A088E7Y6
• Locus tag: MsedB_RS06610
• Protein ID: WP_012021252.1
• Coordinates: 1286424..1286813 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MsedB_RS06615
• Protein ID: WP_012021253.1
• Coordinates: 1286810..1287070 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MsedB_RS06590 (MsedB_1323)	1282160..1283341	+	1182	WP_012021248.1	mandelate racemase/muconate lactonizing enzyme family protein	-
MsedB_RS06595 (MsedB_1324)	1283410..1284033	-	624	WP_012021249.1	PIG-L family deacetylase	-
MsedB_RS06600 (MsedB_1325)	1284068..1285111	-	1044	WP_012021250.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
MsedB_RS06605 (MsedB_1326)	1285204..1286367	-	1164	WP_012021251.1	glycosyltransferase	-
MsedB_RS06610 (MsedB_1327)	1286424..1286813	-	390	WP_012021252.1	type II toxin-antitoxin system VapC family toxin	Toxin
MsedB_RS06615 (MsedB_1328)	1286810..1287070	-	261	WP_012021253.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MsedB_RS06620 (MsedB_1329)	1287136..1287762	-	627	WP_048060318.1	type 1 glutamine amidotransferase	-
MsedB_RS06625 (MsedB_1330)	1287991..1288641	+	651	WP_012021255.1	cyclase family protein	-
MsedB_RS06630 (MsedB_1331)	1288643..1289152	+	510	WP_012021256.1	OsmC family protein	-
MsedB_RS06635 (MsedB_1332)	1289338..1290690	+	1353	WP_012021257.1	APC family permease	-
MsedB_RS06640 (MsedB_1333)	1290705..1291277	-	573	WP_012021258.1	hypothetical protein	-
MsedB_RS06645 (MsedB_1334)	1291509..1291961	+	453	WP_012021259.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15075.63 Da
• Isoelectric Point: 4.9164

>T56096 WP_012021252.1 NZ_CP012173:c1286813-1286424 [Metallosphaera sedula]
MIFLDANFLVYLNLGVDPVKSFYLRILSNESMGVDPLVLDEVIYVSRKKYGVNFGDTLRFLDELVLPYVVVFPISLREYQ
VSKELITRYSLYPSDAFHVAVMLNNSIKKILTEDSDFEKIKEIERIWIK

Nucleotide

• Length: 390 bp

>T56096 NZ_CP012173:c1286813-1286424 [Metallosphaera sedula]
TTGATATTCTTGGATGCAAACTTCTTGGTGTACCTAAACCTGGGAGTTGACCCGGTCAAGAGCTTCTACCTCAGGATACT
TTCCAATGAGAGTATGGGCGTTGACCCCCTGGTCCTAGACGAGGTGATCTACGTCTCGAGGAAGAAGTATGGCGTGAATT
TCGGCGACACATTGCGATTTCTGGACGAGCTAGTCTTACCTTACGTAGTGGTATTTCCGATCTCCCTGAGGGAGTACCAG
GTTAGCAAGGAGCTCATCACTAGGTATTCCCTCTATCCCTCAGACGCGTTTCACGTGGCAGTAATGCTCAACAACTCCAT
AAAGAAGATTCTCACCGAGGACTCCGACTTCGAGAAGATCAAGGAAATAGAGAGGATCTGGATAAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10073.56 Da
• Isoelectric Point: 4.8329

>AT56096 WP_012021253.1 NZ_CP012173:c1287070-1286810 [Metallosphaera sedula]
MTLRVEVGKKGYMIIPKNVRDLLGINEGDFLELRVENGKIVLEKERKVDLEEVERKFEEHERRIAYARRASLGDLKGVVL
EEEFDD

Nucleotide

• Length: 261 bp

>AT56096 NZ_CP012173:c1287070-1286810 [Metallosphaera sedula]
TTGACTCTTAGGGTAGAGGTAGGAAAGAAGGGTTACATGATAATCCCCAAGAACGTCAGAGACCTGCTCGGCATCAATGA
GGGCGACTTCCTGGAACTCAGGGTAGAGAATGGCAAGATAGTCCTGGAAAAGGAAAGGAAAGTTGATCTGGAGGAGGTGG
AGAGGAAGTTTGAGGAACACGAGAGAAGGATCGCGTATGCAAGAAGGGCCAGCCTTGGAGATTTAAAGGGCGTCGTGTTG
GAGGAGGAGTTTGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A088E7Y6