Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 98791..99530 Replicon plasmid p34998-210.894kb
Accession NZ_CP012169
Organism Enterobacter hormaechei subsp. steigerwaltii strain 34998

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag LI65_RS25120 Protein ID WP_013087172.1
Coordinates 99045..99530 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag LI65_RS25115 Protein ID WP_012540086.1
Coordinates 98791..99057 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI65_RS25080 93824..94228 - 405 WP_000839182.1 IS66 family insertion sequence hypothetical protein -
LI65_RS25085 94312..94557 - 246 Protein_105 N-acetyltransferase -
LI65_RS25090 94682..96433 - 1752 WP_162875198.1 arsenical pump-driving ATPase -
LI65_RS25095 96460..96822 - 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
LI65_RS25100 96898..97443 - 546 WP_013087169.1 sigma-70 family RNA polymerase sigma factor -
LI65_RS25105 97452..98165 - 714 WP_013087170.1 arsenical resistance protein ArsH -
LI65_RS25110 98167..98490 - 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
LI65_RS25115 98791..99057 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
LI65_RS25120 99045..99530 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
LI65_RS25125 99949..101289 + 1341 WP_000589001.1 ISNCY family transposase -
LI65_RS25130 101545..101967 - 423 WP_032072095.1 nickel resistance OB fold protein NcrY -
LI65_RS25135 102129..103259 - 1131 WP_004196366.1 Ni(II)/Co(II) efflux transporter permease subunit NcrC -
LI65_RS25140 103272..103541 - 270 WP_004196355.1 nickel-sensing transcriptional repressor NcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B - 1..210894 210894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T56092 WP_013087172.1 NZ_CP012169:99045-99530 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T56092 NZ_CP012169:99045-99530 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT56092 WP_012540086.1 NZ_CP012169:98791-99057 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT56092 NZ_CP012169:98791-99057 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References