Detailed information of TA system TA056089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4605301..4605917
• Replicon: chromosome
• Accession: NZ_CP012167
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34998

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LI65_RS22845
• Protein ID: WP_015569913.1
• Coordinates: 4605301..4605672 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LI65_RS22850
• Protein ID: WP_015569912.1
• Coordinates: 4605675..4605917 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI65_RS22830	4602801..4603703	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
LI65_RS22835	4603700..4604335	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
LI65_RS22840	4604332..4605261	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
LI65_RS22845	4605301..4605672	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
LI65_RS22850	4605675..4605917	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LI65_RS22855	4606116..4607036	+	921	WP_015569911.1	alpha/beta hydrolase	-
LI65_RS22860	4607045..4607986	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
LI65_RS22865	4608031..4608468	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
LI65_RS22870	4608465..4609346	-	882	WP_003861949.1	virulence factor BrkB family protein	-
LI65_RS22875	4609340..4609939	-	600	WP_017694048.1	glucose-1-phosphatase	-
LI65_RS22880	4610058..4610858	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T56089 WP_015569913.1 NZ_CP012167:c4605672-4605301 [Enterobacter hormaechei subsp. steigerwaltii]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T56089 NZ_CP012167:c4605672-4605301 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT56089 WP_015569912.1 NZ_CP012167:c4605917-4605675 [Enterobacter hormaechei subsp. steigerwaltii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT56089 NZ_CP012167:c4605917-4605675 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3