Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1228290..1228910 Replicon chromosome
Accession NZ_CP012165
Organism Enterobacter hormaechei subsp. xiangfangensis strain 34978

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LI63_RS07250 Protein ID WP_015571250.1
Coordinates 1228290..1228508 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LI63_RS07255 Protein ID WP_006809850.1
Coordinates 1228536..1228910 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI63_RS07220 1224302..1224562 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
LI63_RS07225 1224565..1224705 + 141 WP_032608666.1 type B 50S ribosomal protein L36 -
LI63_RS07230 1224702..1225412 - 711 WP_022650493.1 GNAT family N-acetyltransferase -
LI63_RS07235 1225514..1226974 + 1461 WP_032608667.1 PLP-dependent aminotransferase family protein -
LI63_RS07240 1226946..1227413 - 468 WP_022650495.1 hypothetical protein -
LI63_RS07245 1227530..1228081 - 552 WP_022650496.1 maltose O-acetyltransferase -
LI63_RS07250 1228290..1228508 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
LI63_RS07255 1228536..1228910 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LI63_RS07260 1229421..1232567 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit -
LI63_RS07265 1232590..1233783 - 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T56060 WP_015571250.1 NZ_CP012165:c1228508-1228290 [Enterobacter hormaechei subsp. xiangfangensis]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T56060 NZ_CP012165:c1228508-1228290 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT56060 WP_006809850.1 NZ_CP012165:c1228910-1228536 [Enterobacter hormaechei subsp. xiangfangensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT56060 NZ_CP012165:c1228910-1228536 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References