Detailed information of TA system TA056056

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3425147..3425811
• Replicon: chromosome
• Accession: NZ_CP012162
• Organism: Enterobacter roggenkampii strain 35734

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A157QTP3
• Locus tag: LI67_RS16920
• Protein ID: WP_007869731.1
• Coordinates: 3425485..3425811 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0M7FI99
• Locus tag: LI67_RS16915
• Protein ID: WP_007869729.1
• Coordinates: 3425147..3425464 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI67_RS16875	3421226..3421963	+	738	WP_013098169.1	WYL domain-containing protein	-
LI67_RS16880	3422020..3422292	+	273	WP_044597862.1	hypothetical protein	-
LI67_RS16885	3422372..3422632	+	261	WP_007869721.1	hypothetical protein	-
LI67_RS16890	3422759..3423580	+	822	WP_007869723.1	DUF945 domain-containing protein	-
LI67_RS16895	3423604..3423843	+	240	WP_007869725.1	DUF905 domain-containing protein	-
LI67_RS16900	3423947..3424405	+	459	WP_013098170.1	antirestriction protein	-
LI67_RS16905	3424421..3424897	+	477	WP_013098171.1	RadC family protein	-
LI67_RS16910	3424906..3425133	+	228	WP_013098172.1	DUF987 domain-containing protein	-
LI67_RS16915	3425147..3425464	+	318	WP_007869729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LI67_RS16920	3425485..3425811	+	327	WP_007869731.1	TA system toxin CbtA family protein	Toxin
LI67_RS16925	3426046..3427377	-	1332	WP_044597861.1	type VI secretion system ImpA and VasL domain-containing protein	-
LI67_RS16930	3427479..3429071	-	1593	WP_044597860.1	IS66 family transposase	-
LI67_RS16935	3429102..3429452	-	351	WP_044596438.1	IS66 family insertion sequence element accessory protein TnpB	-
LI67_RS16940	3429449..3429856	-	408	WP_016946740.1	transposase	-
LI67_RS16945	3429984..3430454	-	471	WP_025912748.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tssG / tssF / icmF/tssM	3408049..3443960	35911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12299.25 Da
• Isoelectric Point: 8.9951

>T56056 WP_007869731.1 NZ_CP012162:3425485-3425811 [Enterobacter roggenkampii]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Nucleotide

• Length: 327 bp

>T56056 NZ_CP012162:3425485-3425811 [Enterobacter roggenkampii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCAGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGGC
GCGTTTGCTGGAACAGCACTACGGTCTGACAATAAACGACACCCCATTCAGCGATGAAGTTGTTATACAGCAACATATCG
ATGCCGGTATCACCCTGTCAGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
CGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGAGGGCAAGACAGGCAATAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11917.60 Da
• Isoelectric Point: 7.0264

>AT56056 WP_007869729.1 NZ_CP012162:3425147-3425464 [Enterobacter roggenkampii]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT56056 NZ_CP012162:3425147-3425464 [Enterobacter roggenkampii]
ATGACCCACATCATCTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGGCT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGCCGGAAGCTGGATGACACATTCCCAC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A157QTP3

Antitoxin

• AlphaFold DB: A0A0M7FI99