Detailed information of TA system TA056046

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1001604..1002319
• Replicon: chromosome
• Accession: NZ_CP012162
• Organism: Enterobacter roggenkampii strain 35734

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: LI67_RS25355
• Protein ID: WP_023303149.1
• Coordinates: 1001951..1002319 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: LI67_RS05410
• Protein ID: WP_023303148.1
• Coordinates: 1001604..1001930 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI67_RS05380	996638..997903	+	1266	WP_023303143.1	hypothetical protein	-
LI67_RS05385	998212..998646	+	435	WP_023303144.1	hypothetical protein	-
LI67_RS05390	998705..999544	+	840	WP_023303145.1	hypothetical protein	-
LI67_RS05400	1000234..1001055	+	822	WP_023303146.1	DUF945 domain-containing protein	-
LI67_RS05405	1001121..1001591	+	471	WP_023303147.1	DNA repair protein RadC	-
LI67_RS05410	1001604..1001930	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LI67_RS25355	1001951..1002319	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
LI67_RS05415	1002570..1003247	-	678	WP_044597116.1	type A chloramphenicol O-acetyltransferase	-
LI67_RS05420	1003311..1005242	-	1932	WP_008503256.1	GGDEF domain-containing phosphodiesterase	-
LI67_RS05425	1005260..1005754	-	495	WP_044597115.1	oxidoreductase	-
LI67_RS05430	1006156..1006737	-	582	WP_044597114.1	cytochrome b	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	980686..1013897	33211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T56046 WP_023303149.1 NZ_CP012162:1001951-1002319 [Enterobacter roggenkampii]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T56046 NZ_CP012162:1001951-1002319 [Enterobacter roggenkampii]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT56046 WP_023303148.1 NZ_CP012162:1001604-1001930 [Enterobacter roggenkampii]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT56046 NZ_CP012162:1001604-1001930 [Enterobacter roggenkampii]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA