Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 757569..758263 Replicon chromosome
Accession NZ_CP012152
Organism Anoxybacillus gonensis strain G2

Toxin (Protein)


Gene name mazF Uniprot ID A0A0A2SJ00
Locus tag AFK25_RS04130 Protein ID WP_035067430.1
Coordinates 757877..758263 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A4V3UUW2
Locus tag AFK25_RS04125 Protein ID WP_019418225.1
Coordinates 757569..757877 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK25_RS14755 752755..753052 + 298 Protein_765 transposase -
AFK25_RS04105 753122..753892 - 771 WP_035067436.1 3-hydroxybutyrate dehydrogenase -
AFK25_RS04110 753905..755227 - 1323 WP_019418695.1 GntP family permease -
AFK25_RS04115 755451..756800 - 1350 WP_035067434.1 sigma-54-dependent Fis family transcriptional regulator -
AFK25_RS04120 757026..757313 + 288 WP_081957764.1 transposase -
AFK25_RS04125 757569..757877 + 309 WP_019418225.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AFK25_RS04130 757877..758263 + 387 WP_035067430.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AFK25_RS04135 758747..759661 + 915 WP_035067428.1 EamA family transporter -
AFK25_RS04140 759677..759982 - 306 WP_035067426.1 AzlD domain-containing protein -
AFK25_RS04145 759972..760661 - 690 WP_049720867.1 AzlC family ABC transporter permease -
AFK25_RS15070 760789..760965 + 177 WP_165569765.1 hypothetical protein -
AFK25_RS04150 760962..761717 + 756 WP_035067422.1 serine/threonine-protein kinase -
AFK25_RS04155 761731..762060 - 330 WP_035067420.1 helix-turn-helix domain-containing protein -
AFK25_RS04160 762244..763254 + 1011 WP_035067418.1 asparaginase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-116)

Antitoxin

SpoVT-AbrB domain

(26-71)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14230.33 Da        Isoelectric Point: 5.7544

>T56039 WP_035067430.1 NZ_CP012152:757877-758263 [Anoxybacillus gonensis]
MSADVPERGDFVVLNFNPQAGHEQAGRRNAIVLSPKKFNQATGFIAVCPITTQKKGYPFEVDLPKEGISLGGDGYPITGV
VLTDQIKSLDWKARHLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Download         Length: 387 bp

>T56039 NZ_CP012152:757877-758263 [Anoxybacillus gonensis]
ATGTCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCAATCCACAAGCTGGGCATGAGCAGGCTGGGAG
AAGGAATGCGATTGTTTTATCGCCTAAAAAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCGATTACCACTCAAA
AGAAGGGTTACCCTTTCGAAGTGGATTTACCAAAAGAAGGAATATCTCTTGGTGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACTGATCAAATTAAATCATTGGATTGGAAAGCTCGTCATCTAAAAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAAAATAATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11504.10 Da        Isoelectric Point: 6.4776

>AT56039 WP_019418225.1 NZ_CP012152:757569-757877 [Anoxybacillus gonensis]
MNSKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADQIGIKQGSEIELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Download         Length: 309 bp

>AT56039 NZ_CP012152:757569-757877 [Anoxybacillus gonensis]
ATGAACTCAAAGGAACATTTAGAAAGCAAAATAAACCAAAGTAAAGGAGGAGACTACATGTCTACAGTTGTTGCTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGTTGCTGATCAAATAGGGATTAAACAAGGTTCCGAAATAGAAT
TAAATGTGATTGGAAATGAAGGGATCATCACATTAAAACCAAAAAGAACACGGAAGAAATACACACTGGAAGAACTAATA
TCACAGATTACACCTGAAAATCGACACGAAGAAATTGATTTCGGGATAGAAGGGCGTGAATTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A2SJ00

Antitoxin

Source ID Structure
AlphaFold DB A0A4V3UUW2

References