Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 196710..197335 Replicon plasmid 1205p2
Accession NZ_CP012142
Organism Shigella flexneri 4c strain 1205

Toxin (Protein)


Gene name vapC Uniprot ID Q7BEJ1
Locus tag AD871_RS27820 Protein ID WP_000911311.1
Coordinates 196710..197108 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q7BEJ0
Locus tag AD871_RS27825 Protein ID WP_000450531.1
Coordinates 197108..197335 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD871_RS27790 (AD871_27975) 192240..193190 + 951 WP_015683210.1 virulence factor VirK -
AD871_RS27795 (AD871_27980) 193255..194199 + 945 WP_004996485.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
AD871_RS27800 (AD871_27985) 194353..195276 - 924 Protein_232 IS630 family transposase -
AD871_RS27815 (AD871_28000) 196522..196701 + 180 Protein_234 hypothetical protein -
AD871_RS27820 (AD871_28005) 196710..197108 - 399 WP_000911311.1 type II toxin-antitoxin system VapC family toxin Toxin
AD871_RS27825 (AD871_28010) 197108..197335 - 228 WP_000450531.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..222060 222060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14817.08 Da        Isoelectric Point: 8.5232

>T56024 WP_000911311.1 NZ_CP012142:c197108-196710 [Shigella flexneri 4c]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T56024 NZ_CP012142:c197108-196710 [Shigella flexneri 4c]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAAAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8565.62 Da        Isoelectric Point: 4.7513

>AT56024 WP_000450531.1 NZ_CP012142:c197335-197108 [Shigella flexneri 4c]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSTDFMDNREQPGMQERESF

Download         Length: 228 bp

>AT56024 NZ_CP012142:c197335-197108 [Shigella flexneri 4c]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCACCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAAGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3TND
PDB 5ED0
PDB 5ECW
PDB 5ECD
PDB 5ECY

Antitoxin

Source ID Structure
PDB 3TND
AlphaFold DB Q7BEJ0

References