Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 176108..176667 | Replicon | plasmid 1205p2 |
| Accession | NZ_CP012142 | ||
| Organism | Shigella flexneri 4c strain 1205 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | AD871_RS27675 | Protein ID | WP_000421262.1 |
| Coordinates | 176392..176667 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A822PWA0 |
| Locus tag | AD871_RS27670 | Protein ID | WP_011114774.1 |
| Coordinates | 176108..176392 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AD871_RS27640 (AD871_27825) | 172033..172707 | + | 675 | WP_004967157.1 | IS66-like element accessory protein TnpA | - |
| AD871_RS27645 (AD871_27830) | 172704..173051 | + | 348 | WP_000631708.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| AD871_RS27650 (AD871_27835) | 173071..174672 | + | 1602 | WP_011110603.1 | IS66-like element ISSfl3 family transposase | - |
| AD871_RS27655 (AD871_27840) | 174861..175046 | + | 186 | Protein_207 | ATP-binding protein | - |
| AD871_RS27660 (AD871_27845) | 175146..175355 | - | 210 | Protein_208 | peptidoglycan-binding protein | - |
| AD871_RS27665 (AD871_27850) | 175405..175656 | - | 252 | WP_015683204.1 | transporter | - |
| AD871_RS27670 (AD871_27855) | 176108..176392 | + | 285 | WP_011114774.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| AD871_RS27675 (AD871_27860) | 176392..176667 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AD871_RS29870 | 177646..178599 | + | 954 | Protein_213 | IS66 family transposase | - |
| AD871_RS27710 (AD871_27895) | 179135..179353 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| AD871_RS27715 (AD871_27900) | 179355..179660 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA | 1..222060 | 222060 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 164359..186229 | 21870 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T56022 WP_000421262.1 NZ_CP012142:176392-176667 [Shigella flexneri 4c]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T56022 NZ_CP012142:176392-176667 [Shigella flexneri 4c]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10529.97 Da Isoelectric Point: 6.2150
>AT56022 WP_011114774.1 NZ_CP012142:176108-176392 [Shigella flexneri 4c]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR
Download Length: 285 bp
>AT56022 NZ_CP012142:176108-176392 [Shigella flexneri 4c]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PWA0 |