Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 87355..88112 Replicon plasmid 1205p2
Accession NZ_CP012142
Organism Shigella flexneri 4c strain 1205

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag AD871_RS27090 Protein ID WP_000501974.1
Coordinates 87355..87840 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag AD871_RS27095 Protein ID WP_011114751.1
Coordinates 87828..88112 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD871_RS29770 82879..83303 - 425 Protein_100 hypothetical protein -
AD871_RS27065 (AD871_27250) 83531..84169 - 639 WP_000502854.1 ParB N-terminal domain-containing protein -
AD871_RS29775 84154..84297 - 144 Protein_102 DUF3440 domain-containing protein -
AD871_RS29780 86901..87182 + 282 Protein_105 IS4/IS5 family transposase -
AD871_RS27090 (AD871_27275) 87355..87840 - 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
AD871_RS27095 (AD871_27280) 87828..88112 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
AD871_RS30250 (AD871_27290) 89014..89178 + 165 WP_001346193.1 hypothetical protein -
AD871_RS27110 (AD871_27295) 89432..91033 - 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
AD871_RS27115 (AD871_27300) 91030..91380 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
AD871_RS27120 (AD871_27305) 91377..92051 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..222060 222060
- inside IScluster/Tn - ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 32083..100795 68712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T56021 WP_000501974.1 NZ_CP012142:c87840-87355 [Shigella flexneri 4c]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T56021 NZ_CP012142:c87840-87355 [Shigella flexneri 4c]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT56021 WP_011114751.1 NZ_CP012142:c88112-87828 [Shigella flexneri 4c]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT56021 NZ_CP012142:c88112-87828 [Shigella flexneri 4c]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References