Detailed information of TA system TA056019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 158199..158692
• Replicon: plasmid 1205p1
• Accession: NZ_CP012141
• Organism: Shigella flexneri 4c strain 1205

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: AD871_RS26035
• Protein ID: WP_001230709.1
• Coordinates: 158438..158692 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: AD871_RS26030
• Protein ID: WP_000151456.1
• Coordinates: 158199..158441 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD871_RS26000 (AD871_26170)	153359..154285	-	927	WP_001290416.1	hypothetical protein	-
AD871_RS26005 (AD871_26175)	154517..155686	+	1170	Protein_163	IS1380-like element ISEcp1 family transposase	-
AD871_RS26010 (AD871_26180)	155741..156438	+	698	WP_223367350.1	IS1 family transposase	-
AD871_RS26020 (AD871_26190)	156808..157683	+	876	WP_031942271.1	extended-spectrum class A beta-lactamase CTX-M-123	-
AD871_RS26025 (AD871_26195)	157730..158074	-	345	Protein_166	WbuC family cupin fold metalloprotein	-
AD871_RS26030 (AD871_26200)	158199..158441	-	243	WP_000151456.1	hypothetical protein	Antitoxin
AD871_RS26035 (AD871_26205)	158438..158692	-	255	WP_001230709.1	hypothetical protein	Toxin
AD871_RS26040 (AD871_26210)	158689..159231	-	543	WP_001083904.1	hypothetical protein	-
AD871_RS26045 (AD871_26215)	159335..159850	-	516	WP_001025393.1	J domain-containing protein	-
AD871_RS26050 (AD871_26220)	160254..160583	-	330	WP_000855119.1	hypothetical protein	-
AD871_RS26055 (AD871_26225)	161398..161748	+	351	WP_000493378.1	hypothetical protein	-
AD871_RS26060 (AD871_26230)	161799..162542	+	744	WP_000129823.1	hypothetical protein	-
AD871_RS26065 (AD871_26235)	162539..163315	+	777	WP_000015958.1	site-specific integrase	-
AD871_RS26070 (AD871_26240)	163373..163630	-	258	WP_000764642.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / mph(A) / fosA3 / blaCTX-M-123	-	1..248368	248368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T56019 WP_001230709.1 NZ_CP012141:c158692-158438 [Shigella flexneri 4c]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T56019 NZ_CP012141:c158692-158438 [Shigella flexneri 4c]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT56019 WP_000151456.1 NZ_CP012141:c158441-158199 [Shigella flexneri 4c]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT56019 NZ_CP012141:c158441-158199 [Shigella flexneri 4c]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5