Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 101258..101783 Replicon plasmid 981p1
Accession NZ_CP012138
Organism Shigella flexneri 2a strain 981

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag AD867_RS25655 Protein ID WP_001159860.1
Coordinates 101478..101783 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag AD867_RS25650 Protein ID WP_000813626.1
Coordinates 101258..101476 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD867_RS25600 (AD867_25780) 96984..97169 + 186 Protein_120 ATP-binding protein -
AD867_RS30990 (AD867_25785) 97269..97478 - 210 Protein_121 peptidoglycan-binding protein -
AD867_RS25610 (AD867_25790) 97528..97779 - 252 WP_015683204.1 transporter -
AD867_RS25615 (AD867_25795) 98231..98515 + 285 WP_011114774.1 ribbon-helix-helix domain-containing protein -
AD867_RS25620 (AD867_25800) 98515..98790 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
AD867_RS29545 99769..100722 + 954 Protein_126 IS66 family transposase -
AD867_RS25650 (AD867_25830) 101258..101476 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AD867_RS25655 (AD867_25835) 101478..101783 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
AD867_RS25660 (AD867_25840) 102176..103813 + 1638 WP_000936806.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
AD867_RS25665 (AD867_25845) 104021..104290 + 270 Protein_130 type II toxin-antitoxin system antitoxin YacA -
AD867_RS31645 104290..104459 + 170 Protein_131 type II toxin-antitoxin system toxin YacB -
AD867_RS25670 (AD867_25850) 104542..105132 + 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
AD867_RS25680 (AD867_25860) 105489..105755 + 267 Protein_133 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..144188 144188
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 86482..108606 22124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T55992 WP_001159860.1 NZ_CP012138:101478-101783 [Shigella flexneri 2a]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T55992 NZ_CP012138:101478-101783 [Shigella flexneri 2a]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT55992 WP_000813626.1 NZ_CP012138:101258-101476 [Shigella flexneri 2a]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT55992 NZ_CP012138:101258-101476 [Shigella flexneri 2a]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References