Detailed information of TA system TA055991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 98231..98790
• Replicon: plasmid 981p1
• Accession: NZ_CP012138
• Organism: Shigella flexneri 2a strain 981

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2AJT2
• Locus tag: AD867_RS25620
• Protein ID: WP_000421262.1
• Coordinates: 98515..98790 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A822PWA0
• Locus tag: AD867_RS25615
• Protein ID: WP_011114774.1
• Coordinates: 98231..98515 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD867_RS25585 (AD867_25765)	94156..94830	+	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
AD867_RS25590 (AD867_25770)	94827..95174	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
AD867_RS25595 (AD867_25775)	95194..96795	+	1602	WP_011110603.1	IS66-like element ISSfl3 family transposase	-
AD867_RS25600 (AD867_25780)	96984..97169	+	186	Protein_120	ATP-binding protein	-
AD867_RS30990 (AD867_25785)	97269..97478	-	210	Protein_121	peptidoglycan-binding protein	-
AD867_RS25610 (AD867_25790)	97528..97779	-	252	WP_015683204.1	transporter	-
AD867_RS25615 (AD867_25795)	98231..98515	+	285	WP_011114774.1	ribbon-helix-helix domain-containing protein	Antitoxin
AD867_RS25620 (AD867_25800)	98515..98790	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AD867_RS29545	99769..100722	+	954	Protein_126	IS66 family transposase	-
AD867_RS25650 (AD867_25830)	101258..101476	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	-
AD867_RS25655 (AD867_25835)	101478..101783	+	306	WP_001159860.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA	1..144188	144188
	-	inside	IScluster/Tn	-	ospI / ipaH9.8 / ospG	86482..108606	22124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10766.48 Da
• Isoelectric Point: 9.9324

>T55991 WP_000421262.1 NZ_CP012138:98515-98790 [Shigella flexneri 2a]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T55991 NZ_CP012138:98515-98790 [Shigella flexneri 2a]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10529.97 Da
• Isoelectric Point: 6.2150

>AT55991 WP_011114774.1 NZ_CP012138:98231-98515 [Shigella flexneri 2a]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR

Nucleotide

• Length: 285 bp

>AT55991 NZ_CP012138:98231-98515 [Shigella flexneri 2a]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A822PVM3

Antitoxin

• AlphaFold DB: A0A822PWA0