Detailed information of TA system TA055990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 44778..45553
• Replicon: plasmid 981p1
• Accession: NZ_CP012138
• Organism: Shigella flexneri 2a strain 981

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: AD867_RS25255
• Protein ID: WP_000405245.1
• Coordinates: 44778..45260 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: AD867_RS25260
• Protein ID: WP_010921625.1
• Coordinates: 45251..45553 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD867_RS25230 (AD867_25410)	41193..42884	+	1692	WP_000597733.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
AD867_RS29400	42936..44144	+	1209	Protein_58	IS3 family transposase	-
AD867_RS29405	44187..44710	-	524	Protein_59	IS3-like element ISSfl11 family transposase	-
AD867_RS25255 (AD867_25435)	44778..45260	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
AD867_RS25260 (AD867_25440)	45251..45553	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
AD867_RS25265 (AD867_25445)	45825..46097	+	273	WP_000019158.1	hypothetical protein	-
AD867_RS25270 (AD867_25450)	46078..47286	+	1209	WP_000198552.1	IS91 family transposase	-
AD867_RS30970	47458..47586	+	129	Protein_64	IS5/IS1182 family transposase	-
AD867_RS25275 (AD867_25455)	47735..49189	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
AD867_RS25280 (AD867_25460)	49593..50212	+	620	Protein_66	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA	1..144188	144188
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC2	32082..52571	20489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T55990 WP_000405245.1 NZ_CP012138:c45260-44778 [Shigella flexneri 2a]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T55990 NZ_CP012138:c45260-44778 [Shigella flexneri 2a]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT55990 WP_010921625.1 NZ_CP012138:c45553-45251 [Shigella flexneri 2a]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT55990 NZ_CP012138:c45553-45251 [Shigella flexneri 2a]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8