Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3021147..3021801 Replicon chromosome
Accession NZ_CP012137
Organism Shigella flexneri 2a strain 981

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag AD867_RS16405 Protein ID WP_000244767.1
Coordinates 3021147..3021554 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag AD867_RS16410 Protein ID WP_000354052.1
Coordinates 3021535..3021801 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD867_RS16385 (3017104) 3017104..3018837 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
AD867_RS16390 (3018843) 3018843..3019553 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AD867_RS16395 (3019578) 3019578..3020474 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
AD867_RS16400 (3020586) 3020586..3021107 + 522 WP_001055867.1 flavodoxin FldB -
AD867_RS16405 (3021147) 3021147..3021554 - 408 WP_000244767.1 protein YgfX Toxin
AD867_RS16410 (3021535) 3021535..3021801 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
AD867_RS16415 (3022044) 3022044..3023024 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AD867_RS16420 (3023220) 3023220..3023879 - 660 WP_000250274.1 hemolysin III family protein -
AD867_RS16425 (3024043) 3024043..3024353 - 311 Protein_3012 N(4)-acetylcytidine aminohydrolase -
AD867_RS16430 (3024398) 3024398..3025831 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
AD867_RS16435 (3025888) 3025888..3026631 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T55986 WP_000244767.1 NZ_CP012137:c3021554-3021147 [Shigella flexneri 2a]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T55986 NZ_CP012137:c3021554-3021147 [Shigella flexneri 2a]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT55986 WP_000354052.1 NZ_CP012137:c3021801-3021535 [Shigella flexneri 2a]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT55986 NZ_CP012137:c3021801-3021535 [Shigella flexneri 2a]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References