Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1488856..1489494 Replicon chromosome
Accession NZ_CP012127
Organism Escherichia coli strain DH1Ec169

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ADZ28_RS07390 Protein ID WP_000813794.1
Coordinates 1488856..1489032 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ADZ28_RS07395 Protein ID WP_001270286.1
Coordinates 1489078..1489494 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ28_RS07370 1484475..1485689 - 1215 WP_001326689.1 BenE family transporter YdcO -
ADZ28_RS07375 1485742..1486278 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
ADZ28_RS07380 1486351..1488312 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ADZ28_RS07385 1488404..1488634 - 231 WP_000494244.1 YncJ family protein -
ADZ28_RS07390 1488856..1489032 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ADZ28_RS07395 1489078..1489494 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ADZ28_RS07400 1489573..1490979 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
ADZ28_RS07405 1491224..1492369 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
ADZ28_RS07410 1492387..1493400 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
ADZ28_RS07415 1493401..1494342 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T55924 WP_000813794.1 NZ_CP012127:1488856-1489032 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T55924 NZ_CP012127:1488856-1489032 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT55924 WP_001270286.1 NZ_CP012127:1489078-1489494 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT55924 NZ_CP012127:1489078-1489494 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References