Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3018327..3018981 Replicon chromosome
Accession NZ_CP012126
Organism Escherichia coli strain DH1Ec104

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ADZ27_RS14855 Protein ID WP_000244777.1
Coordinates 3018327..3018734 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ADZ27_RS14860 Protein ID WP_000354046.1
Coordinates 3018715..3018981 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ27_RS14835 3014284..3016017 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ADZ27_RS14840 3016023..3016733 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ADZ27_RS14845 3016758..3017654 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ADZ27_RS14850 3017766..3018287 + 522 WP_001055874.1 flavodoxin FldB -
ADZ27_RS14855 3018327..3018734 - 408 WP_000244777.1 toxin CptA Toxin
ADZ27_RS14860 3018715..3018981 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ADZ27_RS14865 3019224..3020204 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ADZ27_RS14870 3020400..3021059 - 660 WP_000250274.1 hemolysin III family protein -
ADZ27_RS14875 3021223..3021534 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ADZ27_RS14880 3021579..3023012 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ADZ27_RS14885 3023069..3023812 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T55897 WP_000244777.1 NZ_CP012126:c3018734-3018327 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T55897 NZ_CP012126:c3018734-3018327 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT55897 WP_000354046.1 NZ_CP012126:c3018981-3018715 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT55897 NZ_CP012126:c3018981-3018715 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References