Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2755027..2755694 Replicon chromosome
Accession NZ_CP012126
Organism Escherichia coli strain DH1Ec104

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag ADZ27_RS13600 Protein ID WP_001094400.1
Coordinates 2755365..2755694 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag ADZ27_RS13595 Protein ID WP_000072690.1
Coordinates 2755027..2755344 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ27_RS13575 2750079..2751088 - 1010 Protein_2605 arsenic transporter -
ADZ27_RS13580 2751230..2752933 + 1704 WP_000896263.1 hypothetical protein -
ADZ27_RS23910 2753505..2753728 + 224 Protein_2607 DUF905 family protein -
ADZ27_RS13585 2753831..2754289 + 459 WP_000211841.1 antirestriction protein -
ADZ27_RS13590 2754298..2754780 + 483 WP_001407480.1 RadC family protein -
ADZ27_RS23915 2754789..2754989 + 201 WP_001395452.1 DUF987 domain-containing protein -
ADZ27_RS13595 2755027..2755344 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ADZ27_RS13600 2755365..2755694 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
ADZ27_RS22800 2756058..2760638 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T55895 WP_001094400.1 NZ_CP012126:2755365-2755694 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T55895 NZ_CP012126:2755365-2755694 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT55895 WP_000072690.1 NZ_CP012126:2755027-2755344 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT55895 NZ_CP012126:2755027-2755344 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References