Detailed information of TA system TA055746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3144199..3144886
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: Mb1595_RS14920
• Protein ID: WP_003414624.1
• Coordinates: 3144443..3144886 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: Mb1595_RS14915
• Protein ID: WP_003414620.1
• Coordinates: 3144199..3144456 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS14895	3139519..3140373	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
Mb1595_RS14900	3140429..3141592	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
Mb1595_RS14905	3141609..3142823	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
Mb1595_RS14910	3142831..3144072	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
Mb1595_RS14915	3144199..3144456	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
Mb1595_RS14920	3144443..3144886	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
Mb1595_RS14925	3144966..3145628	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
Mb1595_RS14930	3145724..3145915	+	192	WP_023349587.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Mb1595_RS14935	3146247..3147995	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
Mb1595_RS14940	3148091..3148672	+	582	WP_003414644.1	fasciclin domain-containing protein	-
Mb1595_RS14945	3148772..3149038	+	267	WP_031652375.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T55746 WP_003414624.1 NZ_CP012095:3144443-3144886 [Mycobacterium tuberculosis variant bovis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T55746 NZ_CP012095:3144443-3144886 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT55746 WP_003414620.1 NZ_CP012095:3144199-3144456 [Mycobacterium tuberculosis variant bovis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT55746 NZ_CP012095:3144199-3144456 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0