Detailed information of TA system TA055744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3097736..3098340
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: Mb1595_RS14690
• Protein ID: WP_003414492.1
• Coordinates: 3097736..3098128 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: Mb1595_RS14695
• Protein ID: WP_003414495.1
• Coordinates: 3098125..3098340 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS14660	3092886..3093674	-	789	WP_193692467.1	CRISPR-associated endoribonuclease Cas6	-
Mb1595_RS14665	3094008..3094553	-	546	WP_003904931.1	DUF1802 family protein	-
Mb1595_RS14670	3094825..3095709	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
Mb1595_RS14675	3095712..3096599	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
Mb1595_RS14680	3096904..3097449	-	546	WP_010950781.1	DUF1802 family protein	-
Mb1595_RS14685	3097446..3097715	-	270	WP_003414489.1	DUF2277 family protein	-
Mb1595_RS14690	3097736..3098128	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
Mb1595_RS14695	3098125..3098340	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Mb1595_RS14700	3098387..3099136	+	750	WP_003414497.1	enoyl-CoA hydratase	-
Mb1595_RS14705	3099215..3100297	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
Mb1595_RS14710	3100290..3101600	-	1311	WP_031702645.1	ABC transporter substrate-binding protein	-
Mb1595_RS14715	3101603..3102430	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
Mb1595_RS14720	3102427..3103337	-	911	Protein_2878	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T55744 WP_003414492.1 NZ_CP012095:c3098128-3097736 [Mycobacterium tuberculosis variant bovis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T55744 NZ_CP012095:c3098128-3097736 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT55744 WP_003414495.1 NZ_CP012095:c3098340-3098125 [Mycobacterium tuberculosis variant bovis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT55744 NZ_CP012095:c3098340-3098125 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8