Detailed information of TA system TA055742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3071190..3071760
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: Mb1595_RS14540
• Protein ID: WP_003414166.1
• Coordinates: 3071190..3071546 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: Mb1595_RS14545
• Protein ID: WP_003901465.1
• Coordinates: 3071530..3071760 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS14520	3066639..3068327	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
Mb1595_RS14525	3068331..3068657	-	327	WP_003414157.1	hypothetical protein	-
Mb1595_RS14530	3068830..3069420	+	591	WP_031656198.1	DUF3558 family protein	-
Mb1595_RS14535	3069439..3071088	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
Mb1595_RS14540	3071190..3071546	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
Mb1595_RS14545	3071530..3071760	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
Mb1595_RS14550	3071803..3072846	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
Mb1595_RS21820	3073037..3073312	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
Mb1595_RS21825	3073488..3073742	-	255	WP_003917684.1	hypothetical protein	-
Mb1595_RS14565	3073890..3074294	+	405	WP_003414181.1	hypothetical protein	-
Mb1595_RS14570	3074291..3074482	+	192	WP_003414184.1	hypothetical protein	-
Mb1595_RS14575	3074546..3075835	+	1290	Protein_2850	transposase family protein	-
Mb1595_RS14580	3076069..3076326	+	258	WP_003899489.1	hypothetical protein	-
Mb1595_RS14585	3076431..3076742	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T55742 WP_003414166.1 NZ_CP012095:c3071546-3071190 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T55742 NZ_CP012095:c3071546-3071190 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT55742 WP_003901465.1 NZ_CP012095:c3071760-3071530 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT55742 NZ_CP012095:c3071760-3071530 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3