55741

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3031602..3032289 Replicon chromosome
Accession NZ_CP012095
Organism Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag Mb1595_RS14325 Protein ID WP_003414064.1
Coordinates 3031894..3032289 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag Mb1595_RS14320 Protein ID WP_003414061.1
Coordinates 3031602..3031868 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mb1595_RS14295 3027241..3028143 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
Mb1595_RS14300 3028212..3028964 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
Mb1595_RS14305 3029208..3029483 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
Mb1595_RS14310 3029480..3031102 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
Mb1595_RS14315 3031189..3031605 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
Mb1595_RS14320 3031602..3031868 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
Mb1595_RS14325 3031894..3032289 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
Mb1595_RS14330 3032286..3032555 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
Mb1595_RS14335 3032565..3033659 - 1095 WP_003414068.1 restriction endonuclease subunit S -
Mb1595_RS14340 3033656..3034075 - 420 Protein_2804 winged helix-turn-helix domain-containing protein -
Mb1595_RS14345 3034149..3034628 - 480 WP_003414073.1 dihydrofolate reductase -
Mb1595_RS14350 3034699..3035499 - 801 WP_003911953.1 thymidylate synthase -
Mb1595_RS14355 3035655..3036392 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T55741 WP_003414064.1 NZ_CP012095:c3032289-3031894 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T55741 NZ_CP012095:c3032289-3031894 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT55741 WP_003414061.1 NZ_CP012095:c3031868-3031602 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT55741 NZ_CP012095:c3031868-3031602 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References