Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2841624..2842261 Replicon chromosome
Accession NZ_CP012095
Organism Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag Mb1595_RS13330 Protein ID WP_003413180.1
Coordinates 2841624..2842019 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag Mb1595_RS13335 Protein ID WP_003413183.1
Coordinates 2842016..2842261 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mb1595_RS13290 2837027..2838238 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
Mb1595_RS13295 2838365..2839024 + 660 WP_031702625.1 LppA family lipoprotein -
Mb1595_RS13300 2839021..2839683 + 663 WP_023643170.1 hypothetical protein -
Mb1595_RS13305 2839680..2839958 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
Mb1595_RS13310 2840051..2840464 + 414 WP_003413164.1 PIN domain nuclease -
Mb1595_RS13315 2840503..2840760 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
Mb1595_RS13320 2840757..2841134 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
Mb1595_RS13325 2841150..2841524 - 375 WP_003413177.1 hypothetical protein -
Mb1595_RS13330 2841624..2842019 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
Mb1595_RS13335 2842016..2842261 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
Mb1595_RS13340 2842672..2843091 - 420 WP_003413190.1 A24 family peptidase -
Mb1595_RS13345 2843103..2843930 - 828 WP_010950736.1 shikimate dehydrogenase -
Mb1595_RS13350 2843908..2845161 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
Mb1595_RS13355 2845154..2845666 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T55738 WP_003413180.1 NZ_CP012095:c2842019-2841624 [Mycobacterium tuberculosis variant bovis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T55738 NZ_CP012095:c2842019-2841624 [Mycobacterium tuberculosis variant bovis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT55738 WP_003413183.1 NZ_CP012095:c2842261-2842016 [Mycobacterium tuberculosis variant bovis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT55738 NZ_CP012095:c2842261-2842016 [Mycobacterium tuberculosis variant bovis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References