Detailed information of TA system TA055729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2241926..2242552
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: Mb1595_RS10475
• Protein ID: WP_003410075.1
• Coordinates: 2242154..2242552 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Mb1595_RS10470
• Protein ID: WP_019283586.1
• Coordinates: 2241926..2242153 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS10460	2239965..2240309	-	345	WP_003410065.1	ferredoxin family protein	-
Mb1595_RS10465	2240498..2241667	-	1170	WP_003899126.1	ATP-binding protein	-
Mb1595_RS10470	2241926..2242153	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
Mb1595_RS10475	2242154..2242552	+	399	WP_003410075.1	PIN domain nuclease	Toxin
Mb1595_RS10480	2242735..2243166	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
Mb1595_RS10485	2243267..2243701	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
Mb1595_RS20925	2244138..2244317	-	180	Protein_2053	hypothetical protein	-
Mb1595_RS10490	2244378..2245025	+	648	WP_080728017.1	transposase	-
Mb1595_RS10495	2244979..2245569	+	591	WP_043856401.1	IS110 family transposase	-
Mb1595_RS10500	2245697..2246953	-	1257	WP_010950642.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T55729 WP_003410075.1 NZ_CP012095:2242154-2242552 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T55729 NZ_CP012095:2242154-2242552 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT55729 WP_019283586.1 NZ_CP012095:2241926-2242153 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT55729 NZ_CP012095:2241926-2242153 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG