Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2218186..2218772 Replicon chromosome
Accession NZ_CP012095
Organism Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag Mb1595_RS10375 Protein ID WP_003410010.1
Coordinates 2218186..2218530 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag Mb1595_RS10380 Protein ID WP_003410014.1
Coordinates 2218524..2218772 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mb1595_RS10340 2213892..2214491 + 600 WP_003409989.1 L-lysine exporter -
Mb1595_RS10345 2214907..2215335 + 429 WP_003409992.1 cellulose-binding protein -
Mb1595_RS10350 2215561..2216100 + 540 WP_031702531.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
Mb1595_RS10355 2216620..2217180 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
Mb1595_RS10360 2217177..2217518 - 342 WP_003410003.1 DUF2384 domain-containing protein -
Mb1595_RS10365 2217604..2217861 + 258 WP_003410006.1 hypothetical protein -
Mb1595_RS10370 2217762..2218097 - 336 WP_003410009.1 dehydrogenase -
Mb1595_RS10375 2218186..2218530 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
Mb1595_RS10380 2218524..2218772 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
Mb1595_RS10385 2218872..2221187 - 2316 WP_003899120.1 cation transporter ATPase CptG -
Mb1595_RS10390 2221184..2221456 - 273 WP_003410017.1 DUF1490 family protein -
Mb1595_RS10395 2221509..2221865 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
Mb1595_RS10400 2222022..2222789 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T55728 WP_003410010.1 NZ_CP012095:c2218530-2218186 [Mycobacterium tuberculosis variant bovis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T55728 NZ_CP012095:c2218530-2218186 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT55728 WP_003410014.1 NZ_CP012095:c2218772-2218524 [Mycobacterium tuberculosis variant bovis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT55728 NZ_CP012095:c2218772-2218524 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References