Detailed information of TA system TA055723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2191243..2191946
• Replicon: chromosome
• Accession: NZ_CP012095
• Organism: Mycobacterium tuberculosis variant bovis strain 1595

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: Mb1595_RS10160
• Protein ID: WP_003409778.1
• Coordinates: 2191243..2191572 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: Mb1595_RS20900
• Protein ID: WP_003409780.1
• Coordinates: 2191569..2191946 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mb1595_RS10140	2187626..2188696	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
Mb1595_RS10145	2188693..2189208	+	516	WP_003409718.1	flavin reductase family protein	-
Mb1595_RS10150	2189205..2190266	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
Mb1595_RS10155	2190263..2191033	+	771	WP_003409775.1	SDR family oxidoreductase	-
Mb1595_RS10160	2191243..2191572	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Mb1595_RS20900	2191569..2191946	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
Mb1595_RS10170	2191943..2192533	-	591	WP_003409784.1	SEC-C domain-containing protein	-
Mb1595_RS10175	2192588..2193952	+	1365	WP_003899094.1	HNH endonuclease	-
Mb1595_RS10180	2194107..2194559	-	453	WP_010950636.1	lipoprotein	-
Mb1595_RS10185	2194623..2195024	+	402	WP_003409869.1	hypothetical protein	-
Mb1595_RS10190	2195017..2195199	-	183	WP_003409870.1	hypothetical protein	-
Mb1595_RS10195	2195313..2195663	-	351	WP_003409871.1	hypothetical protein	-
Mb1595_RS10200	2195674..2196576	-	903	WP_003409874.1	hypothetical protein	-
Mb1595_RS10205	2196597..2196788	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T55723 WP_003409778.1 NZ_CP012095:c2191572-2191243 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T55723 NZ_CP012095:c2191572-2191243 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT55723 WP_003409780.1 NZ_CP012095:c2191946-2191569 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT55723 NZ_CP012095:c2191946-2191569 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8