Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3182612..3183243 Replicon chromosome
Accession NZ_CP012090
Organism Mycobacterium tuberculosis W-148

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag TBPG_RS14885 Protein ID WP_003405820.1
Coordinates 3182932..3183243 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag TBPG_RS14880 Protein ID WP_003405836.1
Coordinates 3182612..3182932 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBPG_RS14850 3177808..3178446 + 639 WP_003898730.1 lipid droplet-associated protein -
TBPG_RS14855 3178443..3179690 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
TBPG_RS14860 3179680..3179937 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
TBPG_RS14865 3179947..3181059 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
TBPG_RS21555 3181077..3182602 - 1526 Protein_2922 carboxylesterase/lipase family protein -
TBPG_RS14880 3182612..3182932 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
TBPG_RS14885 3182932..3183243 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
TBPG_RS14890 3183355..3184512 + 1158 WP_003898727.1 AI-2E family transporter -
TBPG_RS14895 3184519..3185163 - 645 WP_003915494.1 DUF4245 domain-containing protein -
TBPG_RS14900 3185219..3186307 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
TBPG_RS14905 3186338..3187762 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T55679 WP_003405820.1 NZ_CP012090:3182932-3183243 [Mycobacterium tuberculosis W-148]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T55679 NZ_CP012090:3182932-3183243 [Mycobacterium tuberculosis W-148]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT55679 WP_003405836.1 NZ_CP012090:3182612-3182932 [Mycobacterium tuberculosis W-148]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT55679 NZ_CP012090:3182612-3182932 [Mycobacterium tuberculosis W-148]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References