Detailed information of TA system TA055678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3173919..3174487
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TBPG_RS14825
• Protein ID: WP_003405865.1
• Coordinates: 3173919..3174293 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TBPG_RS14830
• Protein ID: WP_003405863.1
• Coordinates: 3174290..3174487 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS14790	3170332..3171039	+	708	Protein_2906	adenylate/guanylate cyclase domain-containing protein	-
TBPG_RS22580	3170991..3171200	-	210	WP_003911400.1	hypothetical protein	-
TBPG_RS14800	3171222..3171932	+	711	WP_003911399.1	hypothetical protein	-
TBPG_RS14805	3171947..3172270	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TBPG_RS14810	3172513..3172788	+	276	WP_003405867.1	hypothetical protein	-
TBPG_RS14815	3172715..3172900	-	186	WP_003901093.1	hypothetical protein	-
TBPG_RS14820	3173018..3173716	-	699	WP_003898733.1	hypothetical protein	-
TBPG_RS14825	3173919..3174293	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBPG_RS14830	3174290..3174487	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBPG_RS14835	3174575..3175648	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TBPG_RS14840	3175711..3176694	+	984	WP_003898731.1	hypothetical protein	-
TBPG_RS14845	3176711..3177718	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TBPG_RS14850	3177808..3178446	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T55678 WP_003405865.1 NZ_CP012090:c3174293-3173919 [Mycobacterium tuberculosis W-148]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T55678 NZ_CP012090:c3174293-3173919 [Mycobacterium tuberculosis W-148]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT55678 WP_003405863.1 NZ_CP012090:c3174487-3174290 [Mycobacterium tuberculosis W-148]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT55678 NZ_CP012090:c3174487-3174290 [Mycobacterium tuberculosis W-148]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07