Detailed information of TA system TA055675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 2905038..2905642
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: TBPG_RS13685
• Protein ID: WP_003414492.1
• Coordinates: 2905038..2905430 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: TBPG_RS13690
• Protein ID: WP_003414495.1
• Coordinates: 2905427..2905642 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS13655	2900188..2900976	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
TBPG_RS13660	2901310..2901855	-	546	WP_014584866.1	DUF1802 family protein	-
TBPG_RS13665	2902127..2903011	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
TBPG_RS13670	2903014..2903901	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
TBPG_RS13675	2904206..2904751	-	546	WP_003910939.1	DUF1802 family protein	-
TBPG_RS13680	2904748..2905017	-	270	WP_003414489.1	DUF2277 family protein	-
TBPG_RS13685	2905038..2905430	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBPG_RS13690	2905427..2905642	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TBPG_RS13695	2905689..2906438	+	750	WP_003902358.1	enoyl-CoA hydratase	-
TBPG_RS13700	2906517..2907599	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
TBPG_RS13705	2907592..2908902	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
TBPG_RS13710	2908905..2909732	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
TBPG_RS13715	2909729..2910640	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T55675 WP_003414492.1 NZ_CP012090:c2905430-2905038 [Mycobacterium tuberculosis W-148]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T55675 NZ_CP012090:c2905430-2905038 [Mycobacterium tuberculosis W-148]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT55675 WP_003414495.1 NZ_CP012090:c2905642-2905427 [Mycobacterium tuberculosis W-148]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT55675 NZ_CP012090:c2905642-2905427 [Mycobacterium tuberculosis W-148]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8