Detailed information of TA system TA055671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2844843..2845522
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: TBPG_RS13335
• Protein ID: WP_003414059.1
• Coordinates: 2844843..2845259 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TBPG_RS13340
• Protein ID: WP_003414061.1
• Coordinates: 2845256..2845522 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS13315	2840895..2841797	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
TBPG_RS13320	2841866..2842618	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TBPG_RS13325	2842862..2843137	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TBPG_RS13330	2843134..2844756	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
TBPG_RS13335	2844843..2845259	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
TBPG_RS13340	2845256..2845522	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBPG_RS13345	2845548..2845943	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
TBPG_RS13350	2845940..2846209	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TBPG_RS13355	2846219..2847313	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
TBPG_RS13360	2847310..2847729	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TBPG_RS13365	2847803..2848282	-	480	WP_003414073.1	dihydrofolate reductase	-
TBPG_RS13370	2848353..2849153	-	801	WP_003911953.1	thymidylate synthase	-
TBPG_RS13375	2849309..2850046	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T55671 WP_003414059.1 NZ_CP012090:c2845259-2844843 [Mycobacterium tuberculosis W-148]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T55671 NZ_CP012090:c2845259-2844843 [Mycobacterium tuberculosis W-148]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT55671 WP_003414061.1 NZ_CP012090:c2845522-2845256 [Mycobacterium tuberculosis W-148]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT55671 NZ_CP012090:c2845522-2845256 [Mycobacterium tuberculosis W-148]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64