Detailed information of TA system TA055667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2643030..2643661
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: TBPG_RS12255
• Protein ID: WP_003413174.1
• Coordinates: 2643284..2643661 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: TBPG_RS12250
• Protein ID: WP_003413167.1
• Coordinates: 2643030..2643287 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS12225	2638945..2639259	+	315	WP_009937839.1	hypothetical protein	-
TBPG_RS12230	2639555..2640766	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
TBPG_RS12235	2640893..2641552	+	660	WP_003902296.1	hypothetical protein	-
TBPG_RS12240	2641549..2642211	+	663	WP_003905878.1	hypothetical protein	-
TBPG_RS22190	2642208..2642485	+	278	Protein_2401	type II toxin-antitoxin system VapB family antitoxin	-
TBPG_RS12245	2642578..2642991	+	414	WP_003413164.1	PIN domain nuclease	-
TBPG_RS12250	2643030..2643287	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
TBPG_RS12255	2643284..2643661	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBPG_RS12260	2643677..2644051	-	375	WP_003413177.1	hypothetical protein	-
TBPG_RS12265	2644151..2644546	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
TBPG_RS12270	2644543..2644788	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
TBPG_RS12275	2645199..2645618	-	420	WP_003413190.1	A24 family peptidase	-
TBPG_RS12280	2645630..2646439	-	810	WP_003413193.1	shikimate dehydrogenase	-
TBPG_RS12285	2646436..2647689	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
TBPG_RS12290	2647682..2648194	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T55667 WP_003413174.1 NZ_CP012090:2643284-2643661 [Mycobacterium tuberculosis W-148]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T55667 NZ_CP012090:2643284-2643661 [Mycobacterium tuberculosis W-148]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT55667 WP_003413167.1 NZ_CP012090:2643030-2643287 [Mycobacterium tuberculosis W-148]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT55667 NZ_CP012090:2643030-2643287 [Mycobacterium tuberculosis W-148]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5