Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2004093..2004679 Replicon chromosome
Accession NZ_CP012090
Organism Mycobacterium tuberculosis W-148

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag TBPG_RS09235 Protein ID WP_003410010.1
Coordinates 2004093..2004437 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag TBPG_RS09240 Protein ID WP_003410014.1
Coordinates 2004431..2004679 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBPG_RS09200 1999799..2000398 + 600 WP_003910883.1 L-lysine exporter -
TBPG_RS09205 2000814..2001242 + 429 WP_003409992.1 cellulose-binding protein -
TBPG_RS09210 2001468..2002007 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
TBPG_RS09215 2002527..2003087 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
TBPG_RS09220 2003084..2003425 - 342 WP_003410003.1 DUF2384 domain-containing protein -
TBPG_RS09225 2003511..2003768 + 258 WP_003410006.1 hypothetical protein -
TBPG_RS09230 2003669..2004004 - 336 WP_003410009.1 dehydrogenase -
TBPG_RS09235 2004093..2004437 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
TBPG_RS09240 2004431..2004679 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
TBPG_RS09245 2004779..2007094 - 2316 WP_003899120.1 cation transporter ATPase CptG -
TBPG_RS09250 2007091..2007363 - 273 WP_003410017.1 DUF1490 family protein -
TBPG_RS09255 2007416..2007772 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
TBPG_RS09260 2007929..2008695 + 767 Protein_1824 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T55658 WP_003410010.1 NZ_CP012090:c2004437-2004093 [Mycobacterium tuberculosis W-148]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T55658 NZ_CP012090:c2004437-2004093 [Mycobacterium tuberculosis W-148]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT55658 WP_003410014.1 NZ_CP012090:c2004679-2004431 [Mycobacterium tuberculosis W-148]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT55658 NZ_CP012090:c2004679-2004431 [Mycobacterium tuberculosis W-148]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References