Detailed information of TA system TA055636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 362152..362795
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB8
• Locus tag: TBPG_RS01580
• Protein ID: WP_003401566.1
• Coordinates: 362370..362795 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: TBPG_RS01575
• Protein ID: WP_003401563.1
• Coordinates: 362152..362373 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS01550	357271..358074	-	804	WP_003401540.1	hypothetical protein	-
TBPG_RS01555	358084..359481	-	1398	WP_003401544.1	sulfatase	-
TBPG_RS01560	359660..361435	+	1776	WP_010886074.1	PE family protein	-
TBPG_RS01565	361578..361805	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
TBPG_RS01570	361802..362104	+	303	WP_003401560.1	toxin-antitoxin system toxin	-
TBPG_RS01575	362152..362373	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
TBPG_RS01580	362370..362795	+	426	WP_003401566.1	PIN domain nuclease	Toxin
TBPG_RS01585	362931..363563	+	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
TBPG_RS01590	363560..364468	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15713.90 Da
• Isoelectric Point: 5.6002

>T55636 WP_003401566.1 NZ_CP012090:362370-362795 [Mycobacterium tuberculosis W-148]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T55636 NZ_CP012090:362370-362795 [Mycobacterium tuberculosis W-148]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT55636 WP_003401563.1 NZ_CP012090:362152-362373 [Mycobacterium tuberculosis W-148]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT55636 NZ_CP012090:362152-362373 [Mycobacterium tuberculosis W-148]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• PDB: 3H87

Antitoxin

• AlphaFold DB: A0A829CG48