Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1574055..1574717 Replicon chromosome
Accession NZ_CP012047
Organism Tetragenococcus halophilus strain MJ4

Toxin (Protein)


Gene name hicA Uniprot ID A0A851I0Q0
Locus tag AC806_RS07425 Protein ID WP_041591998.1
Coordinates 1574532..1574717 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3G5FKS5
Locus tag AC806_RS07420 Protein ID WP_041591845.1
Coordinates 1574055..1574498 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC806_RS07400 1569596..1570474 - 879 WP_061841384.1 NAD(P)-dependent oxidoreductase -
AC806_RS11875 1570560..1570721 - 162 WP_014125287.1 hypothetical protein -
AC806_RS07405 1570770..1572023 - 1254 WP_061841385.1 ABC transporter permease -
AC806_RS07410 1572016..1572915 - 900 WP_014125289.1 ABC transporter ATP-binding protein -
AC806_RS07415 1573104..1573826 - 723 WP_069029099.1 alpha/beta hydrolase -
AC806_RS07420 1574055..1574498 - 444 WP_041591845.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AC806_RS07425 1574532..1574717 - 186 WP_041591998.1 type II toxin-antitoxin system HicA family toxin Toxin
AC806_RS07430 1574822..1575346 - 525 WP_014125293.1 acyl-CoA thioesterase -
AC806_RS07435 1575668..1576453 - 786 WP_014125294.1 acetyl-CoA carboxylase carboxyl transferase subunit alpha -
AC806_RS07440 1576455..1577318 - 864 WP_014125295.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -
AC806_RS07445 1577331..1578695 - 1365 WP_014125296.1 acetyl-CoA carboxylase biotin carboxylase subunit -
AC806_RS07450 1578703..1579122 - 420 WP_180991148.1 3-hydroxyacyl-ACP dehydratase FabZ -
AC806_RS07455 1579134..1579637 - 504 WP_014125298.1 acetyl-CoA carboxylase biotin carboxyl carrier protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(13-80)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 7004.41 Da        Isoelectric Point: 10.7944

>T55566 WP_041591998.1 NZ_CP012047:c1574717-1574532 [Tetragenococcus halophilus]
MPMTQKQMVKYLAKNGWTIVPKQGKGLHVKMCKSNSRPIIIFHGELNKYTERGILKQAGLY

Download         Length: 186 bp

>T55566 NZ_CP012047:c1574717-1574532 [Tetragenococcus halophilus]
ATGCCGATGACACAAAAGCAAATGGTCAAATATCTGGCCAAAAATGGTTGGACAATTGTTCCTAAACAAGGAAAAGGATT
ACATGTTAAAATGTGCAAATCAAATAGTCGTCCAATCATTATTTTTCATGGTGAATTGAACAAATATACCGAAAGAGGAA
TTTTAAAGCAAGCTGGGCTTTATTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16704.72 Da        Isoelectric Point: 4.2592

>AT55566 WP_041591845.1 NZ_CP012047:c1574498-1574055 [Tetragenococcus halophilus]
MLVAYPALFYYDASAQAKYFVTFPDFENSATQGKNIQDALEMASEYLGIIVADLIDTQNHVPSSSDINILDLKENDPFKN
DDDFQLEYDPEKSFITMIYVNINNYLGSQNPVKKTLTIPKWADDLGKKHSLNFSKTLEDAIVEKVNH

Download         Length: 444 bp

>AT55566 NZ_CP012047:c1574498-1574055 [Tetragenococcus halophilus]
ATGTTAGTCGCTTATCCTGCTTTATTTTATTATGACGCTTCAGCTCAAGCAAAATATTTTGTTACTTTTCCAGATTTTGA
AAATAGCGCTACTCAAGGGAAAAATATTCAAGACGCACTTGAGATGGCTTCAGAATACCTGGGGATCATTGTTGCTGATT
TGATTGATACACAGAATCATGTTCCAAGTTCCTCAGATATCAATATACTTGATTTAAAAGAAAACGATCCTTTTAAAAAT
GATGATGATTTCCAATTGGAATATGATCCAGAAAAGTCTTTTATTACGATGATATATGTAAACATAAATAACTATTTAGG
TTCACAAAATCCTGTTAAAAAAACTTTAACGATCCCTAAATGGGCAGATGATTTAGGTAAGAAGCACTCTCTTAATTTCT
CAAAAACATTAGAGGATGCTATTGTAGAAAAAGTGAATCATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A851I0Q0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FKS5

References