Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 469813..470377 Replicon chromosome
Accession NZ_CP012047
Organism Tetragenococcus halophilus strain MJ4

Toxin (Protein)


Gene name mazF Uniprot ID A0A2H6CPR8
Locus tag AC806_RS02220 Protein ID WP_014123850.1
Coordinates 470042..470377 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3G5FHJ2
Locus tag AC806_RS02215 Protein ID WP_041591760.1
Coordinates 469813..470049 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC806_RS02195 465657..467312 + 1656 WP_069028687.1 PTS transporter subunit EIIC -
AC806_RS02200 467309..468064 + 756 WP_083207547.1 HAD-IIB family hydrolase -
AC806_RS02205 468110..468877 - 768 WP_069028689.1 MurR/RpiR family transcriptional regulator -
AC806_RS02210 469203..469304 - 102 WP_069028690.1 putative holin-like toxin -
AC806_RS02215 469813..470049 + 237 WP_041591760.1 hypothetical protein Antitoxin
AC806_RS02220 470042..470377 + 336 WP_014123850.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AC806_RS02225 470651..471202 + 552 WP_069028691.1 GNAT family N-acetyltransferase -
AC806_RS11995 471267..471634 + 368 Protein_451 zeta toxin family protein -
AC806_RS02235 471903..472766 - 864 WP_069029370.1 glycine betaine ABC transporter substrate-binding protein -
AC806_RS02240 472816..473376 - 561 WP_014123854.1 hypothetical protein -
AC806_RS02245 473619..475088 + 1470 WP_061840057.1 betaine-aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12890.98 Da        Isoelectric Point: 8.9118

>T55564 WP_014123850.1 NZ_CP012047:470042-470377 [Tetragenococcus halophilus]
MTNHYTPEKGDIVWIDFDPSTGMEIQKRRPALVISRGNFNTATKFAVVCPITSTEIDLPTRYSLSENYKTKGQIVIHQMK
SLDFNKRKIDFIEKMNPVDLKKIEQIIEFII

Download         Length: 336 bp

>T55564 NZ_CP012047:470042-470377 [Tetragenococcus halophilus]
ATGACTAATCACTATACACCAGAAAAAGGAGACATAGTGTGGATCGACTTTGATCCTTCAACTGGAATGGAAATTCAAAA
GCGACGGCCAGCTCTGGTAATTTCGCGCGGAAATTTTAATACGGCGACAAAATTTGCGGTGGTATGTCCTATTACATCCA
CTGAAATAGATTTACCAACTAGATATAGTCTTTCAGAAAATTATAAAACAAAAGGACAAATAGTCATCCATCAAATGAAA
TCTTTAGATTTCAATAAACGCAAAATTGACTTTATTGAGAAAATGAACCCTGTGGACTTGAAGAAAATAGAACAGATCAT
TGAGTTCATTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8506.60 Da        Isoelectric Point: 4.0338

>AT55564 WP_041591760.1 NZ_CP012047:469813-470049 [Tetragenococcus halophilus]
MLKTKTRAQGRSIVVTLPAEDGATVFPGKEYLVSYGNDGTIVLIPKIEDPFANVTEGAFYESDVWEDMPAVGKEVLDD

Download         Length: 237 bp

>AT55564 NZ_CP012047:469813-470049 [Tetragenococcus halophilus]
ATGCTAAAGACAAAAACAAGGGCACAAGGTAGATCTATCGTGGTTACTTTACCAGCTGAGGATGGGGCAACAGTTTTTCC
TGGGAAAGAATATCTTGTTTCATACGGAAATGATGGAACTATTGTTTTGATACCTAAAATTGAAGATCCGTTTGCTAATG
TTACAGAAGGAGCTTTTTACGAATCGGATGTATGGGAAGATATGCCAGCAGTTGGTAAAGAGGTGCTGGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6CPR8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FHJ2

References