Detailed information of TA system TA055552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(antitoxin)
• Location: 642904..643463
• Replicon: chromosome
• Accession: NZ_CP012040
• Organism: Cyclobacterium amurskyense strain KCTC 12363

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CA2015_RS02500
• Protein ID: WP_048640463.1
• Coordinates: 643218..643463 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CA2015_RS02495
• Protein ID: WP_048640462.1
• Coordinates: 642904..643227 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA2015_RS02480	639060..639410	+	351	WP_048640459.1	hypothetical protein	-
CA2015_RS02485	640030..642174	-	2145	WP_048640460.1	right-handed parallel beta-helix repeat-containing protein	-
CA2015_RS02495	642904..643227	-	324	WP_048640462.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
CA2015_RS02500	643218..643463	-	246	WP_048640463.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
CA2015_RS02505	643682..644056	-	375	WP_048640464.1	helix-turn-helix domain-containing protein	-
CA2015_RS02510	644034..644351	-	318	WP_048640465.1	type II toxin-antitoxin system HigB family toxin	-
CA2015_RS02515	644969..646003	+	1035	WP_157470598.1	glycoside hydrolase family protein	-
CA2015_RS02520	646283..647758	+	1476	WP_048644314.1	sulfatase-like hydrolase/transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (4-103)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9481.94 Da
• Isoelectric Point: 5.1957

>T55552 WP_048640463.1 NZ_CP012040:c643463-643218 [Cyclobacterium amurskyense]
MEISVVKIGNSKGIRLSKTLIERYNIKGRIDLILEKDQIVLRPLATPRKGWEKAFKEMNENGDDKLLIDDVFEDDHLDEW
K

Nucleotide

• Length: 246 bp

>T55552 NZ_CP012040:c643463-643218 [Cyclobacterium amurskyense]
ATGGAAATATCAGTGGTGAAAATTGGGAATTCTAAGGGGATCAGACTTAGTAAAACGCTTATAGAGCGCTATAATATTAA
AGGTAGGATTGATTTAATTCTTGAGAAAGATCAAATTGTTCTAAGGCCATTAGCTACTCCCAGAAAAGGTTGGGAAAAAG
CATTTAAAGAAATGAATGAAAATGGAGACGACAAACTTTTGATTGATGATGTCTTTGAAGACGACCACCTAGACGAATGG
AAATAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12435.67 Da
• Isoelectric Point: 9.9498

>AT55552 WP_048640462.1 NZ_CP012040:c643227-642904 [Cyclobacterium amurskyense]
MEIDQYQIILVNQDPTIGSEIKKTRPCVIIYPNEMNKYLRTLVIAPMTTSSKNYPTRIEVKHDNKIGWIVLDQIRTVDKG
RIIRPLGRLSKPEIKELKAIIKETFVD

Nucleotide

• Length: 324 bp

>AT55552 NZ_CP012040:c643227-642904 [Cyclobacterium amurskyense]
ATGGAAATAGACCAATATCAGATCATCTTGGTGAATCAAGATCCAACCATTGGGAGTGAAATAAAAAAGACCAGACCTTG
TGTTATCATCTATCCCAACGAGATGAATAAATACCTCAGGACCCTTGTTATTGCGCCTATGACAACGAGTTCCAAAAACT
ATCCCACCAGAATTGAGGTAAAACATGACAACAAAATTGGTTGGATTGTTCTTGACCAAATTAGAACAGTTGATAAAGGA
CGTATTATAAGACCTTTGGGCCGGTTATCAAAACCAGAAATAAAAGAATTGAAAGCCATTATCAAAGAGACTTTCGTAGA
CTAA