Detailed information of TA system TA055542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 7454..8019
• Replicon: plasmid pDSM4216
• Accession: NZ_CP012025
• Organism: Bacillus smithii strain DSM 4216

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G9QHP7
• Locus tag: BSM4216_RS15845
• Protein ID: WP_003352727.1
• Coordinates: 7690..8019 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BSM4216_RS15840
• Protein ID: WP_081473003.1
• Coordinates: 7454..7693 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSM4216_RS15815	3259..4071	+	813	WP_174521050.1	helix-turn-helix domain-containing protein	-
BSM4216_RS15820	4086..4526	+	441	WP_048624569.1	hypothetical protein	-
BSM4216_RS15825	4682..5230	+	549	WP_048624570.1	helix-turn-helix domain-containing protein	-
BSM4216_RS16925	5422..5598	+	177	WP_169799179.1	antitoxin VbhA family protein	-
BSM4216_RS15830	6202..6723	-	522	WP_048624571.1	tyrosine-type recombinase/integrase	-
BSM4216_RS15840	7454..7693	+	240	WP_081473003.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSM4216_RS15845	7690..8019	+	330	WP_003352727.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BSM4216_RS15850	8303..8614	-	312	Protein_9	IS4 family transposase	-
BSM4216_RS15855	8746..9081	-	336	WP_048624574.1	hypothetical protein	-
BSM4216_RS15860	9096..10004	-	909	WP_048624575.1	hypothetical protein	-
BSM4216_RS15865	10157..10477	+	321	WP_003352856.1	IS66 family insertion sequence hypothetical protein	-
BSM4216_RS15870	10471..10827	+	357	WP_003352857.1	IS66 family insertion sequence element accessory protein TnpB	-
BSM4216_RS15875	10889..12466	+	1578	WP_048623352.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12514	12514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12240.09 Da
• Isoelectric Point: 5.6913

>T55542 WP_003352727.1 NZ_CP012025:7690-8019 [Bacillus smithii]
MTQAPDRGDLVYLNFNPQSGHEQAGRRPGIVLSPKEFNEITRFALICPITNTVKGWPFEVELPEGLAIQGAILTDQVKSL
DWVSRKIQIKDQAPPEIVTECLNKIHTFL

Nucleotide

• Length: 330 bp

>T55542 NZ_CP012025:7690-8019 [Bacillus smithii]
TTGACCCAAGCACCAGACCGTGGGGATTTGGTTTATCTAAATTTCAACCCACAATCTGGACACGAACAAGCAGGACGTCG
CCCAGGTATTGTATTGTCCCCGAAAGAATTTAATGAAATAACACGATTTGCACTTATTTGTCCAATTACAAACACAGTAA
AGGGTTGGCCTTTTGAAGTAGAGCTCCCAGAAGGTTTAGCCATTCAGGGAGCTATTTTGACTGATCAAGTTAAAAGTTTA
GATTGGGTATCCAGAAAGATTCAAATTAAGGATCAAGCACCACCCGAAATTGTAACTGAGTGTTTGAACAAAATACATAC
TTTTTTGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9049.49 Da
• Isoelectric Point: 8.5898

>AT55542 WP_081473003.1 NZ_CP012025:7454-7693 [Bacillus smithii]
MATTVQKWGNSLAVRIPKDVAERVAIHQGSEIELRVIDDKKITLVPKQKKYTLEELLKQAKPENRHKEIDFGIEGNELI

Nucleotide

• Length: 240 bp

>AT55542 NZ_CP012025:7454-7693 [Bacillus smithii]
ATGGCAACAACTGTTCAAAAGTGGGGAAATAGTCTTGCAGTTCGTATCCCTAAAGATGTTGCTGAGCGAGTTGCGATTCA
TCAAGGCTCCGAAATCGAGTTAAGAGTTATAGATGATAAGAAAATCACATTAGTTCCAAAACAGAAGAAATACACGTTAG
AAGAACTTTTAAAGCAAGCAAAGCCGGAAAATCGTCATAAGGAAATTGATTTTGGTATTGAAGGGAATGAATTAATTTGA

Structures

Toxin

• AlphaFold DB: A0A0H4P587