Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3069850..3070486 Replicon chromosome
Accession NZ_CP012024
Organism Bacillus smithii strain DSM 4216

Toxin (Protein)


Gene name mazF Uniprot ID G9QLS7
Locus tag BSM4216_RS14415 Protein ID WP_004439372.1
Coordinates 3069850..3070200 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G9QLS8
Locus tag BSM4216_RS14420 Protein ID WP_004439375.1
Coordinates 3070205..3070486 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSM4216_RS14400 3067254..3068045 - 792 WP_048624150.1 RNA polymerase sigma factor SigB -
BSM4216_RS14405 3068023..3068493 - 471 WP_048624151.1 anti-sigma B factor RsbW -
BSM4216_RS14415 3069850..3070200 - 351 WP_004439372.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSM4216_RS14420 3070205..3070486 - 282 WP_004439375.1 hypothetical protein Antitoxin
BSM4216_RS14425 3070654..3071829 - 1176 WP_048624153.1 alanine racemase -
BSM4216_RS14430 3072006..3073016 - 1011 WP_004439380.1 outer membrane lipoprotein carrier protein LolA -
BSM4216_RS14435 3073198..3073563 - 366 WP_004439383.1 holo-ACP synthase -
BSM4216_RS14440 3073714..3074310 + 597 WP_048624154.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8998

>T55541 WP_004439372.1 NZ_CP012024:c3070200-3069850 [Bacillus smithii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKISHLDEEMMEKVDEALQISLGLIDF

Download         Length: 351 bp

>T55541 NZ_CP012024:c3070200-3069850 [Bacillus smithii]
TTGATTGTTAAGCGTGGTGATGTGTATTTTGCAGACCTTTCCCCTGTTGTCGGCTCGGAGCAAGGCGGAGTGCGTCCAGT
ACTAGTCCTTCAAAACGACATAGGGAACCGGTTTAGTCCCACTGTAATTGTTGCTGCTATCACAGCGCAAATCCAAAAAG
CCAAACTGCCTACACACGTAGAAATTGACGCAAAGCGATATGGTTTTGAGCGGGATTCCGTTATCTTGCTGGAACAAATA
CGCACAATTGACAAACAACGCTTAACGGATAAAATTTCCCATTTAGATGAAGAAATGATGGAAAAAGTGGACGAAGCCTT
GCAAATCAGTTTAGGTCTAATAGACTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10816.20 Da        Isoelectric Point: 5.2428

>AT55541 WP_004439375.1 NZ_CP012024:c3070486-3070205 [Bacillus smithii]
VSESSTTTEILIRLPQQFLNELDGFAEQENVNRNEFIYRATKAYLRERKKRQIRETMRRGYMEMAKINLAIASEAIQAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT55541 NZ_CP012024:c3070486-3070205 [Bacillus smithii]
GTGTCTGAATCCAGCACAACGACAGAAATCTTAATTCGATTGCCGCAGCAATTTTTAAACGAATTAGACGGATTTGCTGA
GCAGGAAAATGTGAATCGCAATGAATTTATTTATCGCGCCACAAAAGCGTATCTTCGAGAACGCAAAAAGCGTCAAATAA
GGGAAACGATGAGACGAGGCTATATGGAAATGGCAAAAATCAATCTTGCTATTGCCTCCGAGGCTATTCAAGCGGAATAT
GAGGCAGAGAATACGGTAGAACGATTAGTAAGTGGGGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H4P758

Antitoxin

Source ID Structure
AlphaFold DB A0A0H4P3J9

References