Detailed information of TA system TA055533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2718160..2719616
• Replicon: chromosome
• Accession: NZ_CP012019
• Organism: Legionella pneumophila subsp. pneumophila strain Toronto-20052

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: lpt_RS12100
• Protein ID: WP_014842352.1
• Coordinates: 2718160..2719011 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: lpt_RS12105
• Protein ID: WP_027265135.1
• Coordinates: 2719008..2719616 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
lpt_RS12070	2713351..2713704	-	354	WP_014842347.1	conjugal transfer transcriptional regulator TraJ	-
lpt_RS12075	2714119..2714463	+	345	WP_014842348.1	TraK family protein	-
lpt_RS12080	2714463..2715188	+	726	WP_014842349.1	conjugal transfer protein TraL	-
lpt_RS12085	2715188..2715628	+	441	WP_014842350.1	conjugal transfer protein TraM	-
lpt_RS12090	2715793..2716827	+	1035	WP_027265137.1	putative DNA binding domain-containing protein	-
lpt_RS16685	2717571..2717651	-	81	WP_072356411.1	transposase	-
lpt_RS16425	2717660..2718079	+	420	Protein_2372	DUF2779 domain-containing protein	-
lpt_RS12100	2718160..2719011	-	852	WP_014842352.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
lpt_RS12105	2719008..2719616	-	609	WP_027265135.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
lpt_RS12110	2719859..2720122	+	264	WP_014842354.1	hypothetical protein	-
lpt_RS12115	2720122..2720772	+	651	WP_014842355.1	DUF3800 domain-containing protein	-
lpt_RS12120	2720921..2723008	+	2088	WP_014842356.1	hypothetical protein	-
lpt_RS12125	2723010..2723390	-	381	WP_014842357.1	hypothetical protein	-
lpt_RS12130	2723610..2724170	-	561	WP_014842358.1	recombinase family protein	-
lpt_RS12135	2724181..2724498	-	318	WP_014842359.1	DUF2442 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2723610..2724170	560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31702.65 Da
• Isoelectric Point: 6.2785

>T55533 WP_014842352.1 NZ_CP012019:c2719011-2718160 [Legionella pneumophila subsp. pneumophila]
MIKNIGESVRSRLKNIAVKEGSDFNAVLTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSIFADDGISFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLS
DLPAPKIRTYPIYTVIAEKLHAIALLGMANGRLKDYLDLYVLLGNEQIDNQILAKAIQATFNRRGMDLPEALPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T55533 NZ_CP012019:c2719011-2718160 [Legionella pneumophila subsp. pneumophila]
ATGATCAAAAATATTGGTGAGTCCGTACGCTCACGTTTGAAGAATATAGCTGTTAAAGAAGGTTCTGATTTTAATGCGGT
ATTGACTCGTTATGGTCTAGAGCGTTTGTTGTATCGAATTGGAGAATCAGAATATTCTAACCAGTTTTTGTTGAAAGGTG
CCTTACTGTTTAACCTATGGTACGACATGCCCCATCGCCCAACTAAAGATATTGATTTATTGGGATTTGGTGATAACGAT
CTGGCGTATATTAAGCAAACGTTCAGTAAAATTTGCAGCATCTTTGCAGATGATGGAATAAGTTTTCTGTCTGAATCAGT
GACGGTTGACACTATCAAAAAAGATGGAGGTTATACTGGAGCAAGAGTTGAGTTATTTGGGGAATTGGCGAAAGCAAAGA
TTAAAATACAAATTGATATAGGTTATGGTGATGCAGTTACGCCTGGGCCAATAGATGCCCATTATCCCGTACTTCTTAGT
GATCTTCCTGCCCCTAAAATCCGTACTTATCCAATTTATACGGTAATTGCAGAAAAGCTGCACGCAATTGCATTATTGGG
CATGGCGAATGGTCGATTAAAAGACTACTTGGATCTTTATGTATTATTAGGTAATGAACAGATAGATAATCAAATCTTGG
CTAAAGCTATACAAGCCACATTTAATCGTCGAGGTATGGATCTTCCTGAAGCACTGCCTATTGGCCTGACCGATGAGTTT
GCTAACGACCCAAGCAGGGAGTCAATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
CATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22977.01 Da
• Isoelectric Point: 9.6557

>AT55533 WP_027265135.1 NZ_CP012019:c2719616-2719008 [Legionella pneumophila subsp. pneumophila]
MKTEVNNKQKVLQMASKTGVIRMSDLTKKGITRATISRLVSENKLEKLAPGLYCLPETEFSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYNRAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVIVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT55533 NZ_CP012019:c2719616-2719008 [Legionella pneumophila subsp. pneumophila]
ATGAAGACAGAGGTCAACAATAAACAAAAAGTTCTCCAGATGGCTAGTAAAACGGGCGTTATTAGAATGTCCGATCTGAC
CAAGAAGGGGATCACTAGAGCGACCATTTCAAGGCTTGTTAGCGAAAACAAACTTGAAAAACTCGCTCCTGGACTCTATT
GCCTGCCCGAGACTGAGTTTTCAGAAAAGGAAAGCCTGGTTATTATAGCAAGTCGAGTACCTCAAGCAGTGTTTTGCCTG
CTAACTGCTTTGCAGATTCATGATCTAACAACACAGCTACCTCGAAAAGTATGGATAGCTATGCCAAAAGGGAGTCATGC
GCCAAAAATGGATTATCCTCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAACCGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTCATAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA