Detailed information of TA system TA055477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2289188..2289717
• Replicon: chromosome
• Accession: NZ_CP012013
• Organism: Staphylococcus aureus strain Be62

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SABE62_RS11785
• Protein ID: WP_000621175.1
• Coordinates: 2289188..2289550 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SABE62_RS11790
• Protein ID: WP_000948331.1
• Coordinates: 2289547..2289717 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SABE62_RS11760	2286167..2286937	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
SABE62_RS11765	2286912..2287391	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SABE62_RS11770	2287393..2287719	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SABE62_RS11775	2287838..2288839	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SABE62_RS11785	2289188..2289550	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SABE62_RS11790	2289547..2289717	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SABE62_RS11795	2289802..2290950	-	1149	WP_001281145.1	alanine racemase	-
SABE62_RS11800	2291016..2291375	-	360	WP_000581200.1	holo-ACP synthase	-
SABE62_RS11805	2291379..2291870	-	492	WP_001205910.1	PH domain-containing protein	-
SABE62_RS11810	2291857..2293440	-	1584	WP_001294626.1	PH domain-containing protein	-
SABE62_RS11815	2293433..2293912	-	480	WP_001287088.1	hypothetical protein	-
SABE62_RS11820	2294120..2294680	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T55477 WP_000621175.1 NZ_CP012013:c2289550-2289188 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T55477 NZ_CP012013:c2289550-2289188 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT55477 WP_000948331.1 NZ_CP012013:c2289717-2289547 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT55477 NZ_CP012013:c2289717-2289547 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1