Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_XRE(antitoxin)
Location 1168937..1169737 Replicon chromosome
Accession NZ_CP012012
Organism Staphylococcus aureus strain HC1335

Toxin (Protein)


Gene name - Uniprot ID A0A0C6E139
Locus tag SAHC1335_RS05800 Protein ID WP_031767738.1
Coordinates 1168937..1169401 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0C2LD36
Locus tag SAHC1335_RS05805 Protein ID WP_001260487.1
Coordinates 1169414..1169737 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAHC1335_RS05770 1164440..1165579 - 1140 WP_000843611.1 nucleotidyltransferase -
SAHC1335_RS05775 1165706..1166263 + 558 WP_000872158.1 DUF177 domain-containing protein -
SAHC1335_RS05785 1166343..1166516 + 174 WP_000290472.1 50S ribosomal protein L32 -
SAHC1335_RS05790 1166633..1168018 - 1386 WP_000861313.1 recombinase family protein -
SAHC1335_RS05795 1168225..1168905 - 681 WP_000392109.1 type II toxin-antitoxin system PemK/MazF family toxin -
SAHC1335_RS05800 1168937..1169401 - 465 WP_031767738.1 toxin Toxin
SAHC1335_RS05805 1169414..1169737 - 324 WP_001260487.1 helix-turn-helix domain-containing protein Antitoxin
SAHC1335_RS05810 1169901..1170149 + 249 WP_000272859.1 helix-turn-helix domain-containing protein -
SAHC1335_RS05815 1170162..1170608 + 447 WP_031767740.1 hypothetical protein -
SAHC1335_RS05820 1170623..1170766 + 144 WP_000939498.1 hypothetical protein -
SAHC1335_RS05825 1170756..1170965 - 210 WP_000642492.1 hypothetical protein -
SAHC1335_RS05830 1171405..1171590 + 186 WP_000933363.1 helix-turn-helix domain-containing protein -
SAHC1335_RS05835 1171592..1172335 + 744 WP_031767742.1 phage antirepressor KilAC domain-containing protein -
SAHC1335_RS05840 1172511..1172741 - 231 WP_000395455.1 hypothetical protein -
SAHC1335_RS16460 1172791..1172928 + 138 WP_000230552.1 hypothetical protein -
SAHC1335_RS05845 1172921..1173082 + 162 WP_000066026.1 DUF1270 family protein -
SAHC1335_RS05850 1173176..1173436 + 261 WP_000291089.1 DUF1108 family protein -
SAHC1335_RS05855 1173449..1173985 + 537 WP_001004336.1 host-nuclease inhibitor Gam family protein -
SAHC1335_RS05860 1173986..1174633 + 648 WP_020444661.1 ERF family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1166633..1209408 42775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(8-65)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 18142.45 Da        Isoelectric Point: 4.6815

>T55441 WP_031767738.1 NZ_CP012012:c1169401-1168937 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSNFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKLHHID

Download         Length: 465 bp

>T55441 NZ_CP012012:c1169401-1168937 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACACGGCTTTATCTCAGCTGTACCGCTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGCAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCGATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATTACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCATATTGATTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12431.09 Da        Isoelectric Point: 9.3811

>AT55441 WP_001260487.1 NZ_CP012012:c1169737-1169414 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Download         Length: 324 bp

>AT55441 NZ_CP012012:c1169737-1169414 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C6E139

Antitoxin

Source ID Structure
AlphaFold DB A0A0C2LD36

References